基于线粒体DNA 4个基因/区域的矮小梅花鹿群体结构与起源进化分析  

Population Structure and Origin Evolution Analysis of Dwarf Sika Deer Population Based on Four Genes/Regions of Mitochondrial DNA

作　　者：王洪亮[1] 郭肖兰 张然然[1] 王磊[1] 李婷 邢秀梅[1] WANG Hongliang;GUO Xiaolan;ZHANG Ranran;WANG Lei;LI Ting;XING Xiumei(Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;Chinese Medicine Material College, Jilin Agricultural University, Changchun 130118,China)

机构地区：[1]中国农业科学院特产研究所,长春130112 [2]吉林农业大学中药材学院,长春130118

出　　处：《西北农业学报》2018年第12期1723-1730,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：吉林省科技发展计划(20140204058NY)~~

摘　　要：通过线粒体DNA上12S、ND5、Cytb和D-loop 4个基因或区域对分布于吉林省通化地区的矮小梅花鹿群体进行群体结构和起源进化分析,结果表明,各基因在群体中均包括3种单倍型,且每种单倍型个体组成相同。将4个基因整合后形成组合单倍型H1～H3,结合系统发育树判断,H1和H2单倍型与梅花鹿东北亚种聚为一类,而H3单倍型与梅花鹿四川亚种聚为一类,种群历史分析表明,该群体没有经历过扩张。因此认为,该矮小群体有3个母系起源,其中,H1和H2起源于东北亚种,H3可能起源于四川亚种,因为不确定四川亚种群体是否受到东北亚种影响。Based on sequences of four genes or regions of12S,ND5,Cytb and D-loop on mitochondrial DNA,we analyzed the population structure and origin evolution of dwarf sika deer population distributed in Tonghua area of Jilin province.The results indicated that the haplotypes of four genes/region distributed identically in Sika deer population and haplotypes H1to H3were generated by combining four genes/region.Phylogenetic reconstruction analysis indicated H1,H2and Cervus nippon hortulorum subspecies were clustered,while H3and Cervus nippon sichuanicus subspecies were clustered.Analysis of population history showed this population didn’t undergo expansion in history.It was concluded that the dwarf Sika deer population had three maternal origins,H1and H2originated from northeast subspecies,while H3may originated from Sichuan subspecies because we can’t determine if the populations of Sichuan subspecies were affected by northeast subspecies.

关 键 词：矮小梅花鹿 母系遗传 系统发育 单倍型 

分 类 号：S858.25[农业科学—临床兽医学]