控释尿素施用方式对玉米穗位叶氮代谢的影响  

Effects of Different Fertilizer Placement Techniques of Controlled-released Urea on Nitrogen Metabolism in Ear Leaves of Maize

作　　者：聂良鹏 郭利伟[1,2] 宁堂原 吕玉虎[1] 张丽霞 郭晓彦 陈雪青 史鹏飞 张琳 潘兹亮 NIE Liangpeng;GUO Liwei;NING Tangyuan;LV Yuhu;ZHANG Lixia;GUO Xiaoyan;CHEN Xueqing;SHI Pengfei;ZHANG Lin;PAN Ziliang(Xinyang Academy of Agricultural Sciences,Xinyang,Henan 464000,China;State Key Laboratory of Crop Biology,Shandong Key Laboratory of Crop Biology,Shandong Agricultural University,Tai'an,Shandong 271018,China)

机构地区：[1]信阳市农业科学院,河南信阳464000 [2]山东农业大学作物生物学国家重点实验室山东省作物生物学重点实验室,山东泰安271018

出　　处：《天津农业科学》2019年第1期29-33,共5页Tianjin Agricultural Sciences

基　　金：公益性行业(农业)科研专项经费(201503121-05);山东省农业重大应用技术创新项目(2017);信阳市农业科学院科研项目(2018)

摘　　要：试验通过设置4种尿素施用方式,即控释尿素侧施5 cm(C5)和10 cm(C10),控释尿素底施15 cm(CB),普通尿素侧施5 cm(U5),研究其对玉米穗位叶可溶性蛋白和游离氨基酸含量,以及硝酸还原酶、内肽酶和羧肽酶活性的影响。结果表明,与常规尿素相比较,控释尿素深施能够显著提高玉米灌浆中后期穗位叶可溶性蛋白和游离氨基酸含量,以及硝酸还原酶和蛋白水解酶活性;4个处理中以底施效果最优,其次是侧施10 cm。The experiment was conducted to study the effects of different fertilizer placement techniques of controlled-released urea on ear leaf nitrogen metabolism,four fertilization methods were set up,which included the controlled-release urea side application of 5 cm (C5)and 10 cm (C10)depth,bottom dressing of controlled-release urea in 15 cm(CB)depth,and inter-row side dressing of normal urea in 5 cm (U5)depth;soluble protein content,free amino acid content,nitrate reductase activity(NR),endopeptidase activity(EP)and carboxypeptidase activity(CP)were evaluated.The results showed that compared with conventional urea,the application of controlled-released urea could significantly increase the content of soluble protein and free amino acid,as well as the activity of nitrate reductase and proteolytic enzyme in ear leaf of maize grout in the middle and late stage.In the four treatments,the treatment that bottom dressing of controlled-release urea in15 cm(CB)was the best fertilizer placement techniques,the second was the controlled-release urea side application of 10 cm (C10).

关 键 词：玉米 控释尿素 氮代谢 内肽酶 羧肽酶 

分 类 号：S513[农业科学—作物学]