探讨酶联免疫法与胶体金法检测乙肝表面抗原的效果  

To Investigate the Effect of Enzyme-Linked Immunosorbent Assay and Colloidal Gold Assay on Detection of Hepatitis B Surface Antigen

作　　者：王川 程郁菁 雷静 童剑啸 Wang Chuan;Cheng Yu-jing;Lei Jing;Tong Jian-xiao(Sinopharm Wuhan Plasma-derived Biotherapies Co.,Ltd.,Hubei Wuhan 430207)

机构地区：[1]国药集团武汉血液制品有限公司,湖北武汉430207

出　　处：《生物化工》2018年第6期72-74,共3页Biological Chemical Engineering

摘　　要：目的:探讨分析应用酶联免疫法与胶体金法检测乙肝表面抗原的优缺点。方法:选取来自湖北某单采血浆站的76份抗-HBV阳性血清作为研究对象,分别采用酶联免疫法和胶体金法对这些标本进行乙肝表面抗原检测并分析。结果:经胶体金法检测结果及酶联免疫法检测结果同时为阳性的标本有26份。胶体金法检测结果为阳性,而酶联免疫法检测结果为阴性的标本有2份;胶体金法检测结果为阴性,而酶联免疫法检测结果为阳性的标本有3份;胶体金法检测结果及酶联免疫法检测结果同时为阴性的标本有45份。经统计学分析,应用胶体金法进行乙肝表面抗原检测的假阴性率为3.95%,假阳性率为2.63%。应用酶联免疫法进行乙肝表面抗原检测的敏感性明显高于应用胶体金法进行乙肝表面抗原检测的敏感性,差异显著(P <0.05)。结论:酶联免疫法与胶体金法均是检测乙肝表面抗原的比较可靠的方法。胶体金检测法适合于大规模的乙肝病毒快速筛检,酶联免疫检测法则适合于对少数胶体金法检测结果呈阳性的患者进行再次筛检,进而大幅度提升检测的准确率。Objective:To investigate the advantages and disadvantages of using enzyme-linked immunosorbent assay and colloidal gold assay to detect hepatitis B surface antigen.Methods:Seventy-six anti-HBV positive sera from a plasma collection station in Hubei were selected as the study subjects.The specimens were detected and analyzed by enzyme-linked immunosorbent assay and colloidal gold assay.Results There were 26 specimens that were positive by colloidal gold method and enzyme-linked immunosorbent assay.The colloidal gold method showed positive results,while the enzyme-linked immunosorbent assay showed2samples negative;the colloidal gold method showed negative results,while the enzyme-linked immunosorbent assay showed positive samples with 3 samples;colloidal gold detection The results and the results of the enzyme-linked immunosorbent assay were also negative for45samples.After statistical analysis,the false negative rate of hepatitis B surface antigen detection by colloidal gold method was3.95%,and the false positive rate was 2.63%.The sensitivity of detection of hepatitis B surface antigen by enzyme-linked immunosorbent assay was significantly higher than that of the detection of hepatitis B surface antigen by colloidal gold method,and the difference was significant(P<0.05).Conclusion:Both enzyme-linked immunosorbent assay and colloidal gold assay are reliable methods for detecting hepatitis B surface antigen.The colloidal gold detection method is suitable for large-scale rapid screening of hepatitis B virus,and the enzyme-linked immunosorbent assay is suitable for re-screening patients who are positive for a few colloidal gold assays,thereby greatly improving the accuracy of detection.

关 键 词：酶联免疫法 胶体金法 乙肝表面抗原检测 

分 类 号：R446.6[医药卫生—诊断学]