蓝雪花组培快繁无菌体系建立的研究  

Study on Establishment of Sterile System for Tissue Culture and Rapid Propagation of Plumbago auriculata

作　　者：宗树斌[1] 王永平[1] 任焕焕[1] 王静 仕明慧 沈凤 Zong Shubin(Jiangsu Vocational College of Agriculture and Forestry,Jurong 212400,China)

机构地区：[1]江苏农林职业技术学院,江苏句容212400

出　　处：《安徽农学通报》2018年第24期16-18,31,共4页Anhui Agricultural Science Bulletin

基　　金：江苏省林业三新工程项目--林木种苗及植物组培工厂化繁育技术熟化示范(项目编号:LYSX[2016]46);江苏农林职业技术学院院级课题项目--植物种苗工厂化繁育新技术研发及推广(项目编号:2018kj25)

摘　　要：以带腋芽茎段为外植体,通过外植体消毒试验和培养基、激素种类及激素浓度正交试验对蓝雪花组培快繁无菌体系的建立进行研究。结果表明:外植体消毒用10%次氯酸钠灭菌8min,或用0.1%升汞灭菌6min,效果最佳,灭菌充分,致死率低。激素种类因素是影响诱导率的主导因素,试验最佳的组合是WPM培养基+0.4mg/L玉米素(ZT),诱导率达到92.3%。Stem segments with axillary buds were used as explants to study the establishment of the rapid propagation aseptic system of Plumbago auriculata through explant disinfection experiment,and orthogonal experiment of culture medium,hormone types and hormone concentration.The results showed that the best sterilization time of explants with 10% sodium hypochlorite was 8 minutes,the best sterilization time was 6 minutes with 0.1% liter mercury,the best sterilization time was filled with sterilization and mortality rate is low.Orthogonal test showed that hor?mone type was the dominant factor affecting the induction rate,and the best combination was WPM medium+0.4mg/L zeatin(ZT)treatment,the induction rate reached 92.3%.

关 键 词：蓝雪花 外植体 组织培养 

分 类 号：S567[农业科学—中草药栽培]