蛋白质O-GlcNAc糖基化修饰的生物合成途径与检测方法  

Biosynthetic pathways and detection methods of protein O-GlcNAc glycosylation modification

作　　者：李楠 李忻琳 韦立红 林忠 Li Nan;Li Xinlin;Wei Lihong;Lin Zhong(Reproductive Medicine Center,Liuzhou Maternal and Child Healthcare Hospital,Liuzhou 545001,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]柳州市妇幼保健院生殖健康助孕中心,广西壮族自治区545001

出　　处：《中华妇幼临床医学杂志（电子版）》2018年第6期736-739,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：广西壮族自治区自然科学基金资助项目(2015GXNSFBA139177);广西壮族自治区卫生计生委自筹经费科研项目(Z2015180;Z2016558;Z2016560);柳州市科学研究与技术开发计划课题(2015J030515;2016G020217)~~

摘　　要：蛋白质氧连-N-乙酰氨基葡萄糖(O-GlcNAc)糖基化修饰,是一种重要的蛋白质翻译后修饰(PTM),广泛存在于细胞核和细胞质中。蛋白质O-GlcNAc糖基化修饰,由O-GlcNAc转移酶(OGT)和O-GlcNAc水解酶(OGA)共同维持细胞内糖基化水平稳定,动态调节细胞信号转导途径中多种酶的功能,在许多生命活动中发挥着重要调节作用,并与多种代谢性疾病,如妊娠期糖尿病等的发生密切相关。笔者拟就蛋白质O-GlcNAc糖基化的生物合成途径、检测方法的最新研究进展进行综述。Protein O-linked N-acetylglucosamine(O-GlcNAc)glycosylation modification is one of the most important contents of protein post translation modification(PTM).Most of the protein O-GlcNAc glycosylation modifications take place at cell nucleus and cytoplasm.During the process of protein O-GlcNAc glycosylation modification,O-linked N-acetylglucosaminyltransferase(OGT)and O-linked N-acetylglucosaminidase(OGA)together maintain the stability of intracellular glycosylation levels.Protein O-GlcNAc glycosylation modification can dynamically regulate the functions of various enzymes in cell signal transduction pathway,and it plays an important regulatory role in many life activities,and it is closely related to the occurrence of various metabolic diseases such as gestational diabetes.The authors intend to review the latest research progress of the biosynthetic pathways and detection methods of protein O-GlcNAc glycosylation.

关 键 词：糖基化 数据库 蛋白质 氧连-N-乙酰氨基葡萄糖 氧连-N-乙酰氨基葡萄糖转移酶 氧连-N-乙酰氨基葡萄糖水解酶 糖尿病 妊娠 检测方法 

分 类 号：R341[医药卫生—基础医学]