细菌纤维素/胶原蛋白多孔微球的制备及性能  

Preparation and properties of bacterial cellulose/collagen porous microspheres

作　　者：张雯 王学川 强涛涛 李曦月 高畅 ZHANG Wen;WANG Xue-chuan;QIANG Tao-tao;LI Xi-yue;GAO Chang(School of Food and Biological Engineering,Shaanxi University of Science&Technology,Xi'an 710021,China;College of Bioresources Chemical and Materials Engineering,Shaanxi University of Science&Technology,Xi'an 710021,China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]陕西科技大学轻工科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报》2019年第1期18-24,58,共8页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅农业科技攻关计划项目(2016NY-156);西安市未央区科技计划项目(201606)

摘　　要：利用Malaprade反应原理,以高碘酸钠为氧化剂,对细菌纤维素(Bacterial cellulose,BC)进行氧化,制备2,3-二醛细菌纤维素(2,3-dialdehyde bacteria cellulose,DABC).利用希夫碱反应使DABC中的-CHO-与胶原蛋白(collagen,COL)中的-NH2反应,制备CDABC复合材料.采用反相悬浮再生法将CDABC制备成BC/COL多孔微球.以BC/COL多孔微球载药率为指标,采用单因素及正交实验研究CDABC质量分数、十六烷/(CDABC/IL)比例、乳化时间及乳化温度等因素对微球制备影响.利用FT-IR、XRD、TG及SEM对BC、DABC、CDABC、BC/COL多孔微球的化学基团、结晶度、热性能及表面形貌进行分析.结果表明:BC/COL多孔微球制备最优工艺为:乳化温度35℃,乳化时间5h,十六烷∶(CDABC/IL)=10∶1,CDABC质量分数4%.在此工艺下制备BC/COL多孔微球载药率为254 mg/g.FT-IR、XRD、TG及SEM检测结果表明,BC氧化、CDABC复合均能成功进行,且复合作用对材料的形貌、结晶度及热性能均有一定改善作用.采用反相悬浮再生法能够成功制备BC/COL多孔微球,可为后续药物的负载提供条件.In this study,Bacterial cellulose(BC)was oxidized to 2,3-dialdehyde bacteria cellulose(DABC)through Malaprade reaction.Then BC/COL composite materials(CDABC)was prepared by DABC and collagen(COL)through schiff’s base reaction.And BC/COL porous microspheres was prepared with CDABC by reversed-phase suspension regeneration method.During which,with drug-loading rate as an index,CDABC mass fraction,hexadecane/(CDABC/IL)ratio,emulsifying time and temperature were optimized using single factor and orthogonal experiments.The chemical groups,crystallinity,thermal properties and surface morphology of BC,DABC,CDABC,BC/COL porous microspheres were analyzed by FT-IR,XRD,TG and SEM respectively.The results showed that the optimal preparing process of BC/COL porous microspheres was determined as follows:CDABC mass fraction was 4%,hexadecane/(CDABC/IL)ratio was 10:1,emulsifying time was 5 h and emulsifying temperature was 35°C.FT-IR,XRD,TG and SEM results showed that both BC oxidation and CDABC preparation can be successfully carried out,and the morphology,crystallinity and thermal properties of materials can be improved through composition.BC/COL porous microspheres can be prepared successfully by reversed-phase suspension regeneration method,which can provide conditions for subsequent drug loading.

关 键 词：细菌纤维素/胶原蛋白 反相悬浮再生法 多孔微球 

分 类 号：TB332[一般工业技术—材料科学与工程]