一种新型电化学-质谱联用系统的研制  

A Novel System of Electrochemistry Coupled with Mass Spectrometry

作　　者：崔利利 魏忠林[1] 费强[1] 李明[2] CUI Li-Li;WEI Zhong-Lin;FEI Qiang;LI Ming(College of Chemistry,Jilin University,Changchun 130012,China;Division of Chemical Metrology&Analytical Science,National Institute of Metrology,Beijing 100029,China)

机构地区：[1]吉林大学化学学院,长春130012 [2]中国计量科学研究院化学计量与分析科学研究所,北京10029

出　　处：《分析化学》2019年第1期23-29,共7页Chinese Journal of Analytical Chemistry

基　　金：国家重点研发计划(No.2017YFF0205403)资助~~

摘　　要：开发了一种新型电化学与质谱在线联用系统(Coupling electrochemistry with mass spectrometry,EC-MS),采用喷雾针接地的电喷雾离子源作为EC-MS的接口,既避免了离子源的高电压对电化学恒电位仪的损坏,又极大简化了联用仪器结构。为评价该联用系统的性能,考察了氧化型谷胱甘肽(GSSG)的电化学还原行为和还原型谷胱甘肽(GSH)的电化学氧化行为。实验结果表明,此装置具有高灵敏、高反应效率、快速等优点,作为一种新的分析手段,在电化学反应产物和中间体的鉴定、蛋白质组学、药物代谢等方面具有广泛的应用前景。A novel system of electrochemistry coupled with mass spectrometry(EC-MS)was developed.An electrospray ion source with a grounded needle design was used as the interface of the EC-MS,which avoided the damage of the electronic components of the potentiostat and significantly simplified instrument configuration.To evaluate the performance of the novel EC-MS system,the electrochemical reduction behavior of oxidized glutathione(GSSG)and the electrochemical oxidation behavior of reduced glutathione(GSH)were investigated.The experimental result showed that this system had high sensitivity,high reaction efficiency and high speed.As a new analytical approach,it has potential in identification of electrochemical reaction products and intermediates,proteomics and drug metabolism.

关 键 词：电化学-质谱 谷胱甘肽 氧化 还原 

分 类 号：TH843[机械工程—仪器科学与技术] TH832[机械工程—精密仪器及机械]