大黄和水蛭含药血清药物化学实验研究  

Chemical experiment on the serum containing astragalus and rhubarb

作　　者：高旭光 翟文生 杨濛 马静 宋纯东 李广 GAO Xuguang;ZHAI Wensheng;YANG Meng;MA Jing;SONG Chundong;LI Guang(The First Affiliated Hospital of Henan University of TCM,Zhengzhou 450000,China)

机构地区：[1]河南中医医药大学第一附属医院儿科,郑州450000

出　　处：《中国中西医结合儿科学》2018年第6期493-495,553,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目(81273803)

摘　　要：目的检测大黄、水蛭灌胃的大鼠血清中是否含有这两种中药主要成分,为临床用药提供理论依据。方法高效液相色谱法检测大黄组和水蛭组大鼠血清的有效成分大黄酸和大黄素、次黄嘌呤。结果大黄组含有大黄酸和大黄素,虽然大黄酸的检出有一定的干扰,但血清中可以检测到大黄素。水蛭组大鼠血清中检测到次黄嘌呤。结论证实了大鼠血清中含有大黄、水蛭的主要化学成分。Objective To detect whether the serum of the rats irrigated with rhubarb and leech contain the main components of these two drugs,and to provide theoretical basis for clinical use of drugs.Methods The effective components,rhein,emodin and hypoxanthine,in serum of rhubarb group and leech group were determined by HPLC.Results The rat serum of rhubarb group contained rhein and emodin;although the detection of rhein had certain interference,emodin could be detected.Hypoxanthine could be detected in the rat serum of leech group.Conclusion It is confirmed that the main chemical constituents of rhubarb and leech are found in rat serum.

关 键 词：高效液相色谱法 大黄酸 大黄素 次黄嘌呤 

分 类 号：R725.8[医药卫生—儿科]