荧光定量PCR检测生殖道病原体的分子流行病学调查  

Detection and analysis of reproductive tract pathogens by fluorescence quantitative PCR

作　　者：刘玉林[1] 黄晓楠[1] 赵琪林[1] 赵明才[1] LIU Yulin;HUANG Xiaonan;ZHAO Qilin;ZHAO Mingcai(Clinical Laboratory,Central Hospital of Suining,Suining 629000,Sichuang,China)

机构地区：[1]四川省遂宁市中心医院检验科,四川遂宁629000

出　　处：《中国性科学》2018年第12期139-142,共4页Chinese Journal of Human Sexuality

摘　　要：目的:分析遂宁地区2015年至2017年生殖道解脲脲原体(UU)、沙眼衣原体(CT)和淋球菌(NG)感染状况及变化趋势,为地区的疾病防控提供依据。方法:采用实时荧光定量PCR(RT-PCR)技术对3年生殖道标本进行CT、NG、UU病原体DNA检测,并对结果进行统计分析。结果:UU标本3276例(男1209例,女2067例),总阳性率54. 2%(1776/3276)。其中,男性患者阳性标本451例,男性患者阳性检出率37. 3%(451/1209),女性患者阳性标本1325例,女性患者阳性检出率64. 1%(1325/2067),两者差异具有统计学意义(P <0. 05);男女患者皆在21岁～30岁年龄段感染率最高。CT标本2131例(男987例,女1144例),总阳性率14. 7%(313/2131)。其中,男性患者阳性标本187例,男性患者阳性检出率18. 9%(187/987),女性阳性标本126例,女性患者阳性检出率11. 0%(126/1144),两者差异具有统计学意义(P <0. 05);男女患者分别在31岁～40和21岁～30岁年龄段感染率最高。NG标本917例(男605例,女312例),总阳性率16. 2%(149/917)。其中,男性患者阳性标本121例,男性患者阳性检出率20. 0%(121/605),女性患者阳性标本28例,女性患者阳性检出率9. 0%(28/312),两者差异具有统计学意义(P <0. 05),男女患者皆在21岁～30岁年龄段感染率最高。结论:UU是该地区生殖道感染的主要病原体,并以21岁～30岁生殖道感染者阳性率最高,应该重视该群体UU的检测以指导临床医师对患者的诊断和治疗。Objective:To analyze the infection status and variation tendency of ureaplasma urealyticum(UU)、chlamydia trachomatis(CT)and Neisseria gonorrhoeae(NG)in genital tract in Suining city during 2015-2017 to provide the laboratory basis for their diagnosis and treatment.Methods:The real-time fluorescent quantitative PCR(RT-PCR)was applied to detect the CT-DNA,NG-DNA and UU-DNA samples from genital tract and the detection results were performed the statistical analysis.Results:The total positive rate of UU(male 1209,female 2067)was 54.2%(1776/3276);Male patients with positive detection rate is 37.3%(451/1209);Female patients with positive detection rate is 64.1%(1325/2067);The difference was statistically significant(P<0.05);Both male and female at the age of 21-30 ages is the highest infection rate.The total positive rate of CT(male 987,female 1144)was 14.7%(313/2131);Male patients with positive detection rate is 18.9%(187/987);Female patients with positive detection rate 11.0%(126/1144);The difference was statistically significant(P<0.05);Male and female,respectively,at the age of 31-40 and 21-30 ages are the highest infection rate.The total positive rate of NG(male 605,female 312)was 16.2%(149/917);Male patients with positive detection rate is 20.0%(121/605);Female patients with positive detection rate 9.0%(28/312);The difference was statistically significant(P<0.05);Both male and female at the age of 21-30 ages is the highest infection rate.Conclusions:UU is a major pathogen of reproductive tract infections in the region,and the age of 21-30 reproductive tract infection positive rate is highest.The attention should be paid to the group of UU testing to guide clinical doctor to the patient′s diagnosis and treatment.

关 键 词：沙眼衣原体 淋球菌 解脲脲原体 荧光定量PCR 

分 类 号：R446.5[医药卫生—诊断学]