清道夫受体MmSCRA-1调控中红侧沟茧蜂发育的作用研究  

Scavenger receptor MmSCRA-1 regulates the development of endoparasitiod wasps Micropilits mediator

作　　者：周丽贞 王瑞娟 邹振[2] 吕志强[1] ZHOU Li-Zhen;WANG Rui-Juan;ZOU Zhen;LV Zhi-Qiang(College of Plant Protection, Northwest A & F University,Yangling 712100,Shaanxi Province,China;State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences, Beijing 100101,China)

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室,北京100101

出　　处：《环境昆虫学报》2018年第6期1335-1342,共8页Journal of Environmental Entomology

基　　金：陕西省科技攻关项目(2016GYZX17);农业虫害鼠害综合治理研究国家重点实验室开放课题(Chinese IPM1714)

摘　　要：清道夫受体(scavenger receptors,SCRs)是细胞表面的一类跨膜糖蛋白,参与识别多种病原体。中红侧沟茧蜂Micropilits mediator有13个清道夫受体基因,为探究其功能,本文通过系统进化分析,确定与其他物种进化关系,并运用实时荧光定量PCR (qRT-PCR)技术检测中红侧沟茧蜂不同发育时期SCRs基因mRNA的表达水平。选取表达水平较高的A型清道夫受体MmSCRA-1基因进行RNA干扰。结果表明,中红侧沟茧蜂蛹期3日龄注射dsRNA后,dsSCRA-1注射组羽化率显著低于dsGFP注射组(P <0. 05),蛹期3. 5日龄注射dsRNA后,两组羽化率都升高,但dsSCRA-1注射组羽化率仍显著低于dsGFP注射组(P <0. 05)。干扰中红侧沟茧蜂MmSCRA-1基因后,其羽化率降低,说明MmSCRA-1基因在中红侧沟茧蜂蛹期发育过程中起到关键作用。Scavenger receptors (SCRs) are a family of transmembrance glycoproteins that are involved in the recognition of pathogens. Here,we identified and characterized thirteen SCR genes from the endoparasitoid wasp,Micropilits mediator. To explore the function of SCRs in the parasitoid wasp,we analyzed the evolutionary relationship with SCRs from other species,used quantitative Real-Time PCR (qRT-PCR) to analyze transcriptional levels of SCR genes in different developmental stages of M. mediator. The expressional level of MmSCRA-1 gene is higher compared to other MmSCR genes. We knock-downed MmSCRA-1 gene expression using RNA interference. The results showed that eclosion rate of 3-day-old and 3. 5-day-old pupa after MmSCRA-1 gene knockdown were significantly reduced. Altogether,our data demonstrated that MmSCRA-1 might be involved in the development of M. mediator.

关 键 词：中红侧沟茧蜂 清道夫受体 MmSCRA-1 基因 羽化率 

分 类 号：Q963[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]