云南地方绵羊品种遗传多样性分子评价  

Molecular Evaluation of Genetic Diversity of Native Sheep Breeds in Yunnan Province

作　　者：梁家充 朱兰 孙利民 杨红远 袁跃云 李东江 洪琼花 LIANG Jiachong;ZHU Lan;SUN Limin;YANG Hongyuan;YUAN Yueyun;LI Dongjiang;HONG Qionghua(Yunnan Animal Science and Veterinary Institute/Yunnan Provincial Engineering Laboratory of Animal Genetic Resource Conservation and Germplasm Enhancement,Kunming 650224,China;Domestic Animal Breeding and Crossbreed-Improvement Station of Yunnan Province,Kunming 650225,China)

机构地区：[1]云南省畜牧兽医科学院/云南省畜禽遗传资源保护和种质创新工程实验室,昆明650224 [2]云南省家畜改良工作站,昆明650223

出　　处：《四川农业大学学报》2018年第6期822-830,共9页Journal of Sichuan Agricultural University

基　　金：云南省高新技术产业发展项目计划;云南省主要家畜遗传资源保存和选育关键技术研究与应用(云高新产业发展201404)

摘　　要：【目的】利用微卫星标记分析评价8个云南地方绵羊品种的遗传多样性,为资源保护和种质创新提供参考。【方法】提取迪庆绵羊、兰坪乌骨绵羊、宁蒗黑绵羊、石屏青绵羊、腾冲绵羊、昭通绵羊、丽江绵羊和云南半细毛羊这8个云南地方绵羊品种共465只绵羊的血样总DNA,利用10个微卫星标记对这些样本进行遗传多样性,UPGMA系统聚类和群体遗传结构分析。【结果】通过对8个绵羊群体的10个微卫星位点的分析,共发现469个基因型,160个等位基因,平均每个位点有16个等位基因。其中,在位点DYMS1、MAF214和OarFCB 304检测到的等位基因数最多,都为23个;在SRCRP5位点检测到的等位基因数最少,为7个。UPGMA系统聚类图表明,丽江绵羊和宁蒗黑绵羊的遗传距离最近;石屏青绵羊和云南半细毛羊的遗传距离最远。群体遗传结构分析结果显示,465个样本分别来自于8个种群,这与本研究中的绵羊群体来源于8个地方的群体相符。【结论】微卫星位点SRCRSP1、OarFCB304、OarJMP58、MAF33、DYMS1、ILSTS11、MAF214、MCM140和ILSTS28可以作为有效的遗传标记用于绵羊品种的遗传多样性和遗传关系研究。除石屏青绵羊变异最小,其余7个品种都具有较为丰富的遗传多样性,它们之间的遗传分化关系与品种的地域、起源、驯化和相互引种有关。【Objective】The genetic diversity of 8 Yunnan local sheep breeds was evaluated by microsatellite markers in order to provide reference for resource protection and germplasm innovation.【Method】The genetic diversity,UPGMA system cluster and population genetic structure analysis were applied to the total DNA extracts of 465 sheep from eight Yunnan sheep breeds including Diqing sheep,Lanping black bone sheep,Ninglang black sheep,Tengchong sheep,Zhaotong sheep,Lijiang sheep and Yunnan semi-fine wool sheep by analyzing of 10 microsatellite markers.【Result】The results showed that 469 genotype and 160 alleles were found in the analysis of 10 microsatellite loci,and each locus had 16 alleles. The maximum number of alleles was 23,which were found in DYMS1,MAF214 and OarFCB304,On the contrary,only 7 alleles were detected from DYMS1,MAF214 and OarFCB304. UPGMA system analysis results revealed that the genetic distance between Lijiang sheep and Ninglang black sheep was the closest,whereas the furthest genetic distance was found between blue sheep and Shiping Yunnan semi fine wool sheep. The results of population genetic structure analysis indicated that 465 samples were from 8 populations,coincided with the fact that the population of the sheep populations were from 8 places in this study.【Conclusion】These microsatellite loci SRCRSP1, OarFCB304,OarJMP58,MAF33,DYMS1,ILSTS11,MAF214,MCM140 and ILSTS28 can be used as effective markers for studying the genetic diversity of sheep breeds and genetic relationship. Except Shiping indigo sheep,the other seven breeds have abundant genetic diversity. The genetic differentiation among them is related to the region,origin,domestication and mutual introduction of the varieties.

关 键 词：绵羊 微卫星 遗传多样性 

分 类 号：S826.2[农业科学—畜牧学]