PP1γ2对昆明小鼠精子成熟和运动性的调控作用  

Sperm Maturation and Motility in Kunming Mice Regulated by Protein Phosphatase PP1γ2

作　　者：刘芳[1] 杨明华[1] 朱方方[1] 李亚辉[1] LIU Fang;YANG Minghua;ZHU Fangfang;LI Yahui(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2018年第6期1054-1059,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31460308);云南中科灵长类生物医学重点实验室开放基金[云中科字(2016)008号]

摘　　要：【目的】PP1γ2是一种特异表达于动物睾丸和精子的蛋白磷酸酶,为精子发生、运动性获得与调控所必需的关键酶,本文对PP1γ2与昆明小鼠精子成熟和运动性调控进行研究。【方法】通过Western-blot技术,分析昆明小鼠不同条件下附睾头和附睾尾精子中磷酸化和非磷酸化PP1γ2的蛋白质表达量,探讨磷酸酶抑制剂okadaic acid (OA)和cyliculin A (CA)对附睾头和附睾尾精子运动度的影响。【结果】附睾尾精子中磷酸化PP1γ2的蛋白水平远远高于附睾头精子的(P<0.05);db-cAMP、IBMX和Ca^(2+)不改变磷酸化PP1γ2的蛋白水平;OA和CA能显著提高附睾头和附睾尾磷酸化PP1γ2的表达水平,且能显著提高精子(尤其是附睾头的精子)运动度。【结论】PP1γ2通过磷酸化和去磷酸化作用,其酶活性发生变化,从而对昆明小鼠附睾精子成熟和运动性进行调控,即在附睾头精子中PP1γ2酶活性较高,精子运动度较低;在附睾尾精子中PP1γ2酶活性较低,精子运动度得到显著提高。[Purpose]PP1γ2 is a testis-and sperm-specific type of protein phosphatase and it is a key enzyme essential for maturation,initiation and regulation of sperm motility.The regulation of PP1γ2on sperm maturation and motility in Kunming mice was studied.[Method]In the present study,the levels of phosphorylated and dephosphorylated PP1γ2 in caput and caudal epididymal spermatozoa under various conditions were examined via Western-blot,and the effects of two phosphatase inhibitors,i.e.,okadaic acid(OA)and cyliculin A(CA)on motility in caput and caudal epididymal spermatozoa were also investigated.[Result]The level of phosphorylated PP1γ2 was significantly higher(P<0.05)in caudal epididymal spermatozoa than that of caput spermatozoa.The level of phosphorylated PP1γ2 remained unchanged under the treatments of db-cAMP,IBMX or Ca^2+.OA or CA significantly increased not only the level of phosphorylated PP1γ2 in caput and caudal epididymal spermatozoa but also the sperm motility(especially in caput epididymal spermatozoa).[Conclusion]The enzyme activity of PP1γ2 changes with the phosphorylation and dephosphorylation of PP1γ2,thus,PP1γ2 exerts its roles on the regulation of sperm maturation and motility in the epididymis in Kunming mice.To be specific,the high activity of enzyme PP1γ2 in caput epididymal spermatozoa results in lower sperm motility whereas the low activity of enzyme PP1γ2 in caudal epididymal spermatozoa enhances motility significantly.

关 键 词：PP1γ2 精子成熟 运动性 昆明小鼠 附睾 

分 类 号：Q555.4[生物学—生物化学]