顶空—离子分子反应质谱在卷烟真伪鉴别中的应用  

Application of Headspace Coupled with IMR-MS in Discriminating Counterfeit Cigarette from Genuine

作　　者：高莉 王猛 廖头根 朱保昆 马慧宇 李源栋 刘秀明 顾健龙 GAO Li;WANG Meng;LIAO Tougen;ZHU Baokun;MA Huiyu;LI Yuandong;LIU Xiuming;GU Jianlong(Technical Center of China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,China;Yunnan Tobacco Quality Inspection&Supervision Station,Kunming 650106,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,云南昆明650231 [2]云南省烟草质量监督检测站,云南昆明650106

出　　处：《云南农业大学学报（自然科学版）》2018年第6期1099-1104,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南中烟工业有限责任公司科技项目(2015CP04);云南省科技厅青年项目(2017FD237)

摘　　要：【目的】利用顶空—离子分子反应质谱(HS-IMR-MS)对真品卷烟和冒牌卷烟的挥发性成分进行分析,以期为卷烟真伪鉴别提供一种新方法,进一步提高卷烟真伪鉴别的准确性和客观性。【方法】以云烟(软珍品)和云烟(紫)两个牌号各20批次卷烟及各5种冒牌卷烟为试验材料,研究不同电离源、顶空平衡温度及平衡时间对分析效果的影响,通过选取群体特征离子,利用平均法构建两个牌号卷烟的特征质谱指纹图谱,并利用以相关系数法确定的合格阈值及主成分分析,对不同批次真品卷烟及冒牌卷烟进行评价。【结果】(1)以Xe做电离源,顶空平衡温度140℃,顶空平衡时间15 min时,卷烟分析效果最佳;(2)两个牌号不同批次卷烟与其特征质谱指纹图谱的相关系数均在0.99以上,而冒牌卷烟分别为0.914 7～0.934 3和0.884 7～0.908 8,且在主成分分析投影图上,同一牌号卷烟表现为相互聚集。【结论】HS-IMR-MS法能有效区分真品卷烟和冒牌卷烟,可为卷烟真伪鉴别提供新的参考依据。[Purpose]In order to explore a new cigarette determination method and improve the accuracy and objectivity of discriminating counterfeit cigarette from genuine,headspace coupled with ion-molecule reaction mass spectrometry(HS-IMR-MS)was used to detect volatile constituent in genuine cigarettes and counterfeit samples.[Methods]Twenty batches of Yunyan(Soft Rarity)and Yunyan(Purple)respectively and corresponding 5 counterfeit cigarettes respectively were used as the test material.Effects of different types of ionization reagents,balanced temperatures and balanced times on the results were studied,and the representing mass spectrum fingerprints of the2brands were established by getting group characteristic ion peaks with average method.Besides,with the representing mass spectrum fingerprints as reference,different batches of genuine cigarettes and counterfeit samples have been evaluated with principal component analysis(PCA)and qualified thresholds by correlation coefficient.[Results](1)The optimal analysis conditions were selected as following:after balanced at 140℃ for 15min with Xe being the ionization reagents.(2)The correlation coefficients were greater than 0.99 between different batches samples of the two brands respectively and their representing mass spectrum fingerprints.And for counterfeit samples,the correlation coefficients were at 0.9147~0.9343and 0.8847~0.9088.In the PCA projection drawing,the cigarettes were gathered for their brands.[Conclusion]HS-IMR-MS can be a new reference to discriminate counterfeit cigarettes from genuine for quickly and effectively.

关 键 词：离子分子反应质谱 指纹图谱 相似度 主成分分析 假冒卷烟 

分 类 号：TS411[农业科学—烟草工业]