皂树皂苷QS21增强猪支原体肺炎活疫苗滴鼻免疫小鼠效果评价  

Adjuvant QS21 Enhances Immune Responses in Mice Intranasally Immunized Mycoplasma hyopneumoniae Live Vaccine

作　　者：张珍珍[1] 韦艳娜[1] 王佳[1] 于岩飞 冯志新[1] 邵国青[1] 熊祺琰[1] ZHANG Zhen-zhen;WEI Yan-na;WANG Jia;YU Yan-fei;FENG Zhi-xin;SHAO Guo-qing;XIONG Qi-yan(Institute of Veterinary Medicine,Jiangsu Academy of Agriculture Sciences,Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室,南京210014

出　　处：《中国兽药杂志》2018年第12期24-29,共6页Chinese Journal of Veterinary Drug

基　　金：江苏省自然科学基金(BK20160583);国家自然科学基金(31700158)

摘　　要：为提高猪支原体肺炎活疫苗的滴鼻免疫效果,以猪支原体肺炎(168株)活疫苗为抗原,以QS21为免疫佐剂混合后滴鼻免疫小鼠,ELISA法检测免疫效果,包括血清和支气管肺泡灌洗液中抗原特异性抗体水平,并测定了Th1型细胞因子IFN-γ、Th2型细胞因子IL-4和Th17型细胞因子IL-17的分泌情况。结果表明,QS21能激活黏膜和全身性的体液免疫,升高血清和支气管肺泡灌洗液中IgG,IgG1、IgG2a抗体含量,并能提高支气管肺泡灌洗液中黏膜免疫抗体s IgA的含量。QS21还能显著增加支气管肺泡灌洗液中IL-4、IFN-γ和IL-17的分泌,激活Th1、Th2和Th17型细胞免疫应答。以上结果表明QS21能有效激活免疫系统,提示QS21可作为猪肺炎支原体活疫苗的候选黏膜佐剂。To improve intranasally immunized Mycoplasma hyopneumoniae live vaccine,this study used Mycoplasma hyopneumoniae live vaccine(168strains)as antigen,mixed with QS21as immune adjuvant,and then immunized mice by intranasally injection.ELISA method was used to detect immune effect,including the level of antigen-specific antibodies in serum and bronchoalveolar lavage fluid.The Th1type cytokine IFN-γ,Th2 type cytokine IL-4s ecretion and Th17 type cytokines IL-17 were also determined.The results showed that QS21 induced mucosal and systemic humoral immune response.QS21 increased the antibody level of immunized mice,promoted the production of IgG,IgG1,IgG2a antibodies in serum and bronchoalveolar lavage fluid,and increased the level of mucosal immune antibody sIgA.QS21 significantly increased the secretion of IL-4,IFN-γand Th17in bronchoalveolar lavage fluid and increased Th1,Th2 and Th17 type immune responses.The above results indicate that QS21 has great advantages in inducing immune responses and has the prospect of developing as a mucosal candidate adjuvant for the Mycoplasma pneumonia live vaccine.

关 键 词：QS21 佐剂 滴鼻 猪支原体肺炎活疫苗 

分 类 号：S852.65[农业科学—基础兽医学]