基于自然对流PCR与毛细管电泳技术的牙周病原菌检测  

Detection of periodontal pathogen by natural convection PCR and capillary electrophoresis

作　　者：王林燕 陶春先[1] 山口佳则[2] 李振庆[1] WANG Linyan;TAO Chunxian;YAMAGUCHI Yoshinori;LI Zhenqing(School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;Department of Physics, East China University of Science and Technology, Shanghai 200237, China)

机构地区：[1]上海理工大学光电信息与计算机工程学院,上海200093 [2]华东理工大学物理系,上海200237

出　　处：《光学仪器》2018年第6期7-12,共6页Optical Instruments

基　　金：国家自然科学基金(21205078);高等学校博士学科点专项科研基金博导类资助课题(20123120110002)

摘　　要：细菌种类的快速识别在牙病防治中具有重要的应用价值。以牙周病原菌为研究对象,建立了DNA快速扩增的自然对流聚合酶链式反应(PCR)方法及毛细管电泳荧光光学检测系统。研究表明,当毛细管有效长度为8cm、电场强度为100V/cm时,50bp DNA ladder在筛分介质为0.5%HEC(羟乙基纤维素)(1 300K)中的分离效果最佳。毛细管电泳结果表明,采用自然对流法可以在25min内在圆柱腔体中实现牙周病原菌的快速PCR。Rapid identification of bacterial species is of great value in the field of molecular biology.In this work,we built a fast natural convection polymerase chain reaction(PCR) method and confocal fluorescence capillary electrophoresis system for the detection of periodontal pathogens.Result shows that 50 bp DNA ladder can be well resolved in 0.5% HEC(hydroxyethyl cellulose)(1 300 K)when the effective length of the capillary is 8 cm and the electric field strength is 100 V/cm.It also shown that the DNA could be successfully amplified within 25 min by natural convection of PCR.

关 键 词：毛细管电泳系统 聚合酶链式反应(PCR) 自然对流 

分 类 号：O657.7[理学—分析化学]