柴胡皂甙d上调CDKN1B抑制脑胶质瘤细胞增殖的机制研究  

Proliferation Inhibition Mechanism of Saikosaponins-d by Up-Regulating CDKN1B in Glioma Cells

作　　者：马鹏举[1] 李祥生[1] 汲乾坤 刘瑞华[1] 惠磊[1] 金保哲[1] MA Pengju;LI Xiangsheng;JI Qiankun;LIU Ruihua;HUI Lei;JIN Baozhe(The First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China)

机构地区：[1]新乡医学院第一附属医院,河南新乡453100

出　　处：《中医药信息》2019年第1期5-10,共6页Information on Traditional Chinese Medicine

基　　金：新乡医学院第一附属医院青年基金项目(No.QN-2017-B009)

摘　　要：目的:探讨柴胡皂甙d(Saikosaponins-d,SSd)对脑胶质瘤细胞增殖的作用及调控机制。方法:体外培养脑胶质瘤Μ251细胞株,加入终浓度分别为0、5、10、20μg/m L的SSd,采用CCK-8法检测Μ251细胞的增殖率,通过流式细胞仪分析细胞周期及细胞凋亡率的改变,RT-PCR法检测细胞CDKN1B mRNA的表达水平。通过CDKN1B siRNA复原转染实验,以10μg/m L的SSd为对照,检测CDKN1B在SSd影响胶质瘤增殖、细胞周期及凋亡中的作用。结果:SSd可抑制脑胶质瘤Μ251细胞的增殖水平,且随SSd浓度越高,抑制作用越明显。同时SSd阻滞细胞周期在G0/G1期,促进胶质瘤细胞凋亡。CDKN1B siRNA能明显促进胶质瘤的增殖,CDKN1B表达下调能明显恢复SSd抑制的胶质瘤细胞增殖能力,同时能恢复SSd阻滞的胶质瘤细胞周期,抑制胶质瘤细胞的凋亡水平。结论:柴胡皂甙d能够通过抑制增殖并促进凋亡对人脑胶质瘤Μ251细胞生长起到抑制作用,其机制可能与上调CDKN1B的表达有关。Objective: To investigate the effect and mechanism of Saikosaponins-d(SSd) on the cell proliferation glioma cell. Methods: Gliomas U251 cell line was cultured in vitro and the proliferation of U251 cell was detected by CKK-8 method after adding different final concentrations of SSd(0 ug/mL, 5 ug/mL, 10 ug/mL, and 20 ug/mL). Flow cytometry was used to monitor changes in U251 cell cycle and apoptosis. The mRNA expression of CDKN1B was detected by RT-PCR. The effect of CDKN1B on SSd(influencing the proliferation, cell cycle, and apoptosis rates) in U251 cells was observed after transfection with CDKN1B siRNA. Results: With the increasing concentrations of SSd, the proliferation rates decreased remarkedly. The activity of U251 cells was inhibited, the cell cycle was arrested in G0/G1 phase, and the cell apoptosis was increased after using SSd. CDKN1B siRNA could promote the proliferation of UC251, down-expression of CDKN1B restored the proliferation inhibition and G0-G1 phase transition, also inhibited the apoptosis level of UC251 regulated by SSd. Conclusion: SSd may inhibit the cell activity of glioma cell line U251 and promote cell apoptosis in vitro via increasing the CDKN1B expression.

关 键 词：脑胶质瘤 柴胡皂甙D CDKN1B 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]