植物线粒体巯基亚硝基化修饰蛋白质组学研究进展  

Advances on plant mitochondrial S-nitrosylation proteomics

作　　者：张秋楠 喻娟娟 秦智 戴绍军 ZHANG Qiunan;YU Juanjuan;QIN Zhi;DAI Shaojun(Development Center of Plant Germplasm Resources,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Harbin 150040,Heilongjiang,China)

机构地区：[1]上海师范大学生命科学学院植物种质资源开发协同创新中心,上海200234 [2]东北林业大学盐碱地生物资源环境研究中心,黑龙江哈尔滨150040

出　　处：《上海师范大学学报（自然科学版）》2018年第6期647-652,共6页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市科学技术委员会科研计划项目(17391900600);上海市协同创新中心项目(ZF1205)

摘　　要：蛋白质S-亚硝基化是一氧化氮(NO)与蛋白质半胱氨酸残基(Cys)共价连接形成S-亚硝基硫醇(-SNO)的过程,被认为是植物中体现NO生物活性的最重要途径.线粒体在依赖S-亚硝基化的NO信号转导中起关键作用.综述了应用蛋白质组学技术鉴定的植物线粒体S-亚硝基化蛋白质的特征,为认识线粒体NO调控网络体系中重要的信号与代谢通路(如光呼吸、三羧酸循环、氧化磷酸化、活性氧分子(ROS)稳态,以及蛋白质加工与周转)提供了线索.Protein S-nitrosylation is the process in which nitric oxide (NO) moiety is covalently attached to protein cysteine (Cys) to form S-nitrosothiol (-SNO).It is considered to be the most important way to reflect the biological activity of NO in plants.Mitochondria play a key role in NO signaling dependent on S-nitrosylation.Proteomics approach has proven to be a very efficient tool for identifying S-nitrosylated proteins in plants.The findings provide insight into important signaling and metabolic pathways in the NO regulatory network of mitochondria (e.g.,photorespiration,tricarboxylic acid cycle,oxidative phosphorylation,reactive oxygen species (ROS)homeostasis,and protein processing and turnover).

关 键 词：植物 线粒体 S-亚硝基化 蛋白质组学 

分 类 号：Q946.1[生物学—植物学]