不同光照条件下氮浓度对衣藻生长及油脂积累的影响  

Interactive effects of nitrogen concentrations and light intensity on growth and lipid accumulation of Chlamydomonas reinhardtii

作　　者：陈余佳 涂晓盟 王永强 王全喜 许丽丽 CHEN Yujia;TU Xiaomeng;WANG Yongqiang;WANG Quanxi;XU Lili(Development Center of Plant Germplasm Resources,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学生命科学学院植物种质资源开发协同创新中心,上海200234

出　　处：《上海师范大学学报（自然科学版）》2018年第6期697-703,共7页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(31600284);上海市协同创新中心项目(ZF1205);上海植物种质资源工程技术研究中心项目(17DZ2252700)

摘　　要：为探究莱茵衣藻(Chlamydomonas reinhardtii)油脂含量的最佳条件,在不同的光照条件下检测了氮浓度对莱茵衣藻生物量及油脂含量的影响.结果表明:在氮添加和光照强度增加的梯度上,衣藻的油脂含量逐渐降低;光照强度和氮添加量对衣藻的油脂含量都有显著影响;控制光照强度和氮添加量后发现,当光照强度为30μE·m^(-2)·s^(-1),氮质量浓度为100 mg·mL^(-1)时,衣藻油脂含量的质量分数为39.79%,叶绿素质量浓度为17.08 mg·mL^(-1),最适宜衣藻油脂积累.In order to explore the optimum condition of the growth and lipid accumulation of C. reinhardtii ,our study investigated the lipid accumulation of C. reinhardtii across the gradients of nitrogen concentration and light intensity.The results showed that,the lipid accumulation of C.reinhardtii decreased along the gradients of nitrogen concentration and light intensity.Light intensity and nitrogen concentration had significant effects on lipid accumulation of C.reinhardtii .After controlling light intensity and nitrogen concentration,our study showed that the composition of 30 μE·m^-2 ·s^-1 in light intensity and 100 mg·mL^-1 in nitrogen mass concentration were optimum condition of the growth and lipid accumulation.In this case,the lipid content and chlorophyll mass fraction of C.reinhardtii were 39.79% and 17.08 mg·mL^-1 ,respectively.

关 键 词：氮浓度 莱茵衣藻(Chlamydomonas reinhardtii) 油脂 

分 类 号：Q945[生物学—植物学]