药用植物丹参中丹参酮ⅡA的提取分离工艺研究  

Purification of tanshinone ⅡA from medicinal plant Salvia miltiorrhiza

作　　者：张毅 邓昌平 时敏 付蓉 王瑶 曹建国 开国银 ZHANG Yi;DENG Changping;SHI Min;FU Rong;WANG Yao;CAO Jianguo;KAI Guoyin(Development Center of Plant Germplasm Resources,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;College of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 311402,Zhejiang,China)

机构地区：[1]上海师范大学生命科学学院植物种质资源开发协同创新中心,上海200234 [2]浙江中医药大学药学院,浙江杭州311402

出　　处：《上海师范大学学报（自然科学版）》2018年第6期754-761,共8页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(81522049,31270007,31571735,30900110);上海市科学技术委员会项目(17JC1404300,15430502700);上海市曙光学者计划(16SG38);浙江省高校高水平创新团队资助;上海市协同创新中心项目(ZF1205);上海植物种质资源工程技术研究中心项目(17DZ2252700)

摘　　要：石油醚、乙酸乙酯作为萃取剂,利用聚酰胺凝胶柱分离,乙醇/水梯度洗脱以分离丹参(Salvia miltiorrhiza),得到丹参酮ⅡA.所得到的产物经过核磁共振谱(1H NMR、13C NMR)确证了结构,并经薄层色谱(TLC)、高效液相色谱(HPLC)分析,显示其纯度达到93%以上.本研究提供了一种高效的丹参酮ⅡA的提取分离方法,具有提取率高、成本低、安全可靠等优点,也为其他药用植物有效成分的分离提供参考.In this study,petroleum ether and ethyl acetate were used as extraction agents,and polyamide column chromatography was used to separate tanshinone ⅡA using ethanol/water as eluting solvents.TLC, 1H NMR, 13 C NMR,HPLC analysis revealed that the purity of the extracted tanshinone ⅡA was over 93%.Above all,this study provides a highly efficient extraction and separation method for tanshinone ⅡA which displayed a series of advantages including higher yield,lower cost,higher safety and reliability,etc.And it also provides a new approach for purification of other active ingredients from medicinal plants.

关 键 词：丹参(Salvia miltiorrhiza) 丹参酮ⅡA 纯化 聚酰胺 柱层析 

分 类 号：R932[医药卫生—生药学]