杉木育种园的遗传多样性和杂种优势群的划分研究  

Genetic diversity and heterotic grouping of Cunninghamia lanceolata breeding garden

作　　者：齐明[1,2] 王海蓉 彭九生[4] 周建革[3] 翁春媚 何贵平[1,2] Qi Ming;Wang Hairong;Peng Jiusheng;Zhou Jian′ge;Weng Chunmei;He Guiping(Research Institute of Subtropical Forestry of CAF, Fuyang Zhejiang 311400, China;Zhejiang Provincial Key Laboratory of Tree Breeding,Fuyang Zhejiang 311400, China;Forestry Technology Extension Station of Suichang County, Suichang Zhejiang 323300, China;Jiangxi Academy of Forestry, Nanchang Jiangxi 330013, China)

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]浙江省林木育种技术研究重点实验室,浙江富阳311400 [3]遂昌县林业技术推广总站,浙江遂昌323300 [4]江西省林业科学院,江西南昌330013

出　　处：《南方林业科学》2018年第6期17-21,共5页South China Forestry Science

基　　金：浙江省"十三五"林木新品种育种专项(项目编号:2016C02056-5);中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金资助子项(项目编号:CAFYBB2017 ZA001-1-2)

摘　　要：为了了解杉木育种群体的遗传多样性,并合理地划分杉木杂种优势群,以期为杂交亲本选配服务。本研究借助ISSR分子标记技术,对遂昌县1.5代杉木种子园(兼作育种群体)40个亲本的遗传多样性进行了研究,在此基础上,采用DPS软件划分杂种优势群。研究结果发现:(1)杉木育种园育种群体具有较高的遗传多样性,具有较大的改良潜力;(2)根据亲本间的分子遗传距离,杉木育种群体可划分为4个杂种优势群:(3)每个杂种优势群中均有优良的母本或父本,不同杂种优势群中的优良母本和父本进行组配,遂昌县杉木育种园中可获得有希望的优良杂交组合60对;这些拟优良杂交组合中一些组合的速生性,在林业生产实践中得到了印证,例如龙15×1339、1419×1339、1278×1258等组合表现优良,杉木一代杂种龙15×闽33、丽55×丽13也是不同的杂种优势群间产生的。这些拟优良组合为指导杉木的杂交育种提供了科学依据。In order to study genetic diversity of Cunninghamia lanceolata breeding population,reasonably divide heterotic groups of parental population,and provide scientific basis for cross breeding,by means of the technique of molecular marker of ISSR,genetic diversity of40parents in1.5generation of C.lanceolata seed orchard(as breeding group)had been studied,and on this basis,DPS software was used to divide heterotic groups.The results showed as follows:(1)There is higher genetic diversity in breeding population of C.lanceolata,and has great improvement potential;(2)On this basis,cluster analysis was used by molecular genetic distance,breeding population composed of40parent clones has been divided into4heterotic groups at molecular genetic distance equaling to0.60;(3)There were better male or female parents in each different heterotic groups,the matching of fine female and male between different heterotic groups,60promising cross combinations have been produced by parents matching,some excellent cross combinations have been proved in the practice of forestry production,for example:Long15×1339,1419×1339,1278×1258;even one generation hybrid of C.lanceolata:Long15×Min33,Li55×Li13are also produced by different heterotic groups.These Quasi-fine combinations provide a scientific basis for the hybrid breeding of C.lanceolata.

关 键 词：杉木 ISSR标记 遗传多样性 聚类分析 杂种优势群 

分 类 号：S791.27[农业科学—林木遗传育种] S722.3[农业科学—林学]