c-Jun蛋白对A型流感病毒H1N1感染小鼠体内CD4^+和CD8^+T细胞增殖的调节作用  

c-Jun protein effect on regulating CD4^+ and CD8^+ T cell proliferation in H1N1 influenza A virus infected mice

作　　者：张守平[1,2] 申汶涛[1] 王丽荣 张百重[3] ZHANG Shouping;SHEN Wentao;WANG Lirong;ZHANG Baizhong(College of Animal Science and Veterinary Medicine,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China;College of Veterinary Medicine,China Agricultural University,Beijing 100192,China;College of Resources and Environment,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)

机构地区：[1]河南科技学院动物科技学院,河南新乡453003 [2]中国农业大学动物医学院,北京100192 [3]河南科技学院资源与环境学院,河南新乡453003

出　　处：《浙江大学学报（农业与生命科学版）》2018年第6期722-726,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：中国博士后科学基金面上项目(2016M602243);河南省科技攻关计划(农业领域)项目(182102110053;182102110039)

摘　　要：通过构建A型流感病毒H1N1感染小鼠模型,并使用c-Jun蛋白的特异性核酶Dz13抑制该蛋白的表达,探索c-Jun蛋白在流感病毒感染后对T淋巴细胞增殖和炎性反应的调节作用。蛋白质印迹法(Western-blotting)结果表明,核酶Dz13作用后c-Jun蛋白的表达量明显降低,且c-Jun蛋白的活化也随之降低。此外,用流式细胞术测定感染后3 d和6 d外周血内辅助性T细胞(CD4^+T)和杀伤性T细胞(CD8^+T)的增殖情况,同时用实时荧光定量聚合酶链式反应测定外周血内细胞因子IFN-γ、IL-6和IL-10表达。结果显示:小鼠感染病毒后,外周血CD4+和CD8+T细胞明显增多,相关细胞因子的表达量也明显上调;而抑制c-Jun蛋白表达后,CD4^+和CD8^+T细胞的增殖受到抑制,且相关细胞因子IFN-γ、IL-6和IL-10的表达量亦受到抑制。说明c-Jun蛋白参与调节A型流感病毒H1N1感染后引起的T淋巴细胞增殖和相关细胞因子的表达。A H1N1 influenza A virus infected mice model was built to evaluate the regulation mechanism of c-Jun protein in T lymphocytes. To determine the DNAzyme Dz13 efficiency in virus-infected animals, a Westernblotting assay was employed to detected total c-Jun(t-c-Jun) and phosphorylation c-Jun(p-c-Jun) levels by specific antibody. It was demonstrated that the drug-treated mice markedly suppressed the expression of t-c-Jun and p-c-Jun. Through flow cytometry and quantitative real-time polymerase chain reaction(qRT-PCR), we could find an obvious CD4^+(helper T lymphocytes) and CD8^+T(cytotoxic T lymphocyte) cell proliferation and relative cytokine(IFN-γ, IL-6 and IL-10) expression on day 3 and 6 in the virus-infected mice. The Dz13-treated mice with a c-Jun suppression displayed a down-regulation of CD4+and CD8+T cells in peripheral blood. At the same time, Dz13-treated group demonstrated a significant reduction of inflammatory cytokine(IFN-γ, IL-6 and IL-10) expression. In conclusion, c-Jun protein regulates CD4^+ and CD8^+T cell proliferation and inflammatory cytokine expression in H1N1 influenza A virus infected mice.

关 键 词：C-JUN蛋白 A型流感病毒 T淋巴细胞 细胞因子 

分 类 号：Q28[生物学—细胞生物学]