5-氮杂-2’-脱氧胞苷对胎牛成纤维细胞增殖、周期和凋亡的影响  

Effects of 5-azacytidine-2'-deoxycytidine on the proliferation,cell cycle,and apoptosis of fetal bovine fibroblast cells

作　　者：于孟飞 王文璐 易建明[2] YU Mengfei;WANG Wenlu;YI Jianming(Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China/Institute for Medical Biology,College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China;Key Laboratory of Agricultural Animal Genetic,Breeding,and Reproduction of Ministry of Education,College of Animal Science&Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]中南民族大学生命科学学院医学生物研究所/武陵山区特色植物资源种质保护与利用湖北省重点实验室,武汉430074 [2]华中农业大学动物科学技术学院,农业动物遗传育种与繁殖教育部重点实验室,武汉430070

出　　处：《浙江大学学报（农业与生命科学版）》2018年第6期727-734,共8页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：Project supported by the Fundamental Research Funds for the Central Universities(2013PY130);the National Natural Science Foundation of China for Youths(81400252)

摘　　要：胎牛成纤维细胞是细胞重编程和去甲基化研究的常用材料。本文以胎牛成纤维细胞为材料,用不同浓度的DNA去甲基抑制剂药物5-氮杂-2’-脱氧胞苷分别处理24、48和72 h后,研究其对胎牛成纤维细胞的增殖、活力、细胞周期分布和凋亡率的影响。结果显示,5-氮杂-2’-脱氧胞苷以浓度依赖性的方式抑制胎牛成纤维细胞的增殖、活力,并增加细胞的总凋亡率。其中,用浓度低于0.1μmol/L的5-氮杂-2’-脱氧胞苷处理24 h时,胎牛成纤维细胞受到的不利影响最小。这也说明采用相对低浓度的DNA去甲基抑制剂处理体细胞对于细胞的重编程是有益的。Fetal bovine fibroblast cells(FBFCs) are widely used in cellular reprogramming and methylation. In this study, the effects of 5-azacytidine-2’-deoxycytidines(5-Aza-CdR), a DNA methylation inhibitor on the physiological characteristics of FBFCs were investigated. The FBFCs derived from a bovine fetus were treated with 5-Aza-CdR at different concentrations for 24, 48 and 72 h, respectively, to determine the changes of cell proliferation, viability, cell cycle distribution and apoptosis rates. The results demonstrated that 5-Aza-CdR treatment in the FBFCs resulted in a decrease of cell proliferation, cell viability, and an increase of total cell apoptosis in a dose-dependent manner. The best treatment strategy for 5-Aza-CdR was less than 0.1 μmol/L for24 h, which showed minimal adverse effects on FBFCs. The above results indicate that the low dose DNA demethylation inhibitor treatment in FBFCs is beneficial for cell reprogramming.

关 键 词：5-氮杂-2'-脱氧胞苷 细胞增殖 细胞周期 细胞凋亡 

分 类 号：Q253[生物学—细胞生物学] Q28