肾上腺素注射液中亚硫酸氢钠的含量测定  

Determination of sodium bisulfite in Epinephrine Injection

作　　者：汪志强 文瑾[3] 方夏琴 王登才 张宇佳 郑稳生 WANG Zhiqiang;WEN Jin;FANG Xiaqing;WANG Dengcai;ZHANG Yujia;ZHENG Wensheng(Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing City Key Laboratory of Drug Delivery Technology and Novel Formulation,Beijing 100050,China;Anhui University of Chinese Medicine,Hefei 230012,China;Chinese PharmaceuticalAssociation,Beijing 100022,China)

机构地区：[1]中国医学科学院药物研究所,药物传输技术及新型制剂北京市重点实验室,北京100050 [2]安徽中医药大学,安徽合肥230012 [3]中国药学会,北京100022

出　　处：《药学研究》2018年第12期699-701,738,共4页Journal of Pharmaceutical Research

基　　金：中国医学科学院医学与健康科技创新工程项目(No.2017-I2M-1-011)

摘　　要：目的建立肾上腺素注射液中抗氧化剂亚硫酸氢钠的含量测定方法。方法基于在SO2存在下可以使得酸性品红溶液褪色的原理,采用比色法在548 nm波长处测定样品吸光度,根据吸光度值计算抗氧化剂的含量。结果在亚硫酸氢钠浓度为4～24μg·m L-1,吸光度值的倒数与其浓度呈良好的线性关系Y=0.007 8X+1.628 6(R2=0.999 5),平均回收率为102.50%(RSD=2.22%,n=9)。结论该方法准确,简便易行,可用于对肾上腺素注射液中亚硫酸氢钠含量的测定。Objective To develop a method for the determination of sodium bisulfite in Epinephrine Injection. Methods Based on the fading of fuchsin acid solution in the present of SO2 ,a colorimetric method was established to be used for the determination of absorbance at the wavelength of 548 nm,the amount of sodium bisulfite in sample was calculated by the absorbance value. Results The reciprocal of absorbance and the concentrations of sodium sulfite showed a good linear relationship over the range of 4~24 μg·mL -1 , Y =0.007 8 X +1.628 6( R2 =0.999 5) with the average recovery of 102.50%( RSD =2.22%, n =9). Conclusion The method was sample and accurate and can be used for the determination of antioxidant sodium bisulfite in Epinephrine Injection.

关 键 词：肾上腺素注射液 亚硫酸氢钠 含量 比色法 

分 类 号：R927.2[医药卫生—药学]