硝普钠对肉桂酸处理黄瓜和黑籽南瓜根生理特性的影响  

Effects of sodium nitroprusside on root physiological characteristics in cucumber and figleaf gourd seedlings under CA treatment

作　　者：张永平[1] ZHANG Yongping(Department of Life Science,Tangshan Normal University,Tangshan,Hebei 063000,China)

机构地区：[1]唐山师范学院生命科学系,河北唐山063000

出　　处：《西北农林科技大学学报（自然科学版）》2019年第2期97-102,111,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河北省自然科学基金面上项目(C2015105091);唐山师范学院科学研究基金重点项目(2013D06)

摘　　要：【目的】探究硝普钠(SNP,NO供体)预浸种对肉桂酸(CA)处理黄瓜和黑籽南瓜根系生长及根边缘细胞(BCs)生物学特性的影响。【方法】研究不同浓度(0,50,100,200和400μmol/L)的SNP溶液浸种对250μmol/L CA处理黄瓜和黑籽南瓜根长、根系活力及根BCs的数目、存活率及黏胶层厚度的影响。【结果】CA单独处理降低了黄瓜根系长度、BCs数目及其存活率,增加了BCs的黏胶层厚度,但对根系活力影响不显著。50和100μmol/L的SNP能够缓解CA对黄瓜根系长度、BCs数目与存活率的抑制作用,100μmol/L SNP还降低了黄瓜BCs的黏胶层厚度;200和400μmol/L的SNP提高了CA对根长、BCs数目与存活率的抑制作用,并引起BCs黏胶层厚度进一步增加。CA单独处理对黑籽南瓜幼苗根长与根系活力影响不大,但降低了BCs数目与存活率,增加了BCs黏胶层厚度。50和100μmol/L的SNP能够提高CA处理下黑籽南瓜BCs数目与存活率,而BCs黏胶层厚度变化不明显;400μmol/L SNP抑制了黑籽南瓜根系生长,降低了BCs的数目与存活率,但对BCs黏胶层厚度与根系活力影响不明显。【结论】50与100μmol/L SNP溶液浸种均能在一定程度上缓解CA对黄瓜的胁迫作用,以100μmol/L SNP为好;CA对黑籽南瓜胁迫程度较小,SNP对黑籽南瓜受CA胁迫程度的缓解作用也远小于黄瓜。【Objective】The experiment was carried out to study the effects of sodium nitroprusside(SNP)on root growth and border cell(BCs)biological characteristics of cucumber and figleaf gourd seedlings under cinnamic acid(CA)treatment.【Method】The root length,activity,number,viability and mucilage thickness of BCs in cucumber and figleaf gourd soaked with SNP at different concentrations(0,50,100,200 and 400μmol/L)under CA(250μmol/L)treatment were explored.【Result】Root length,number and viabilities of BCs in cucumber treated with CA decreased,mucilage thickness of BCs increased,and root activities had no significant change.SNP soaking with concentrations of 50 and 100μmol/L alleviated inhibition of CA on root length,number and viability of BCs in cucumber,and 100μmol/L SNP also decreased mucilage thickness of BCs.SNP soaking at concentrations of 200 and 400μmol/L enhanced the inhibitory effect of CA on root length,number and viability of BCs in cucumber,and further increased mucilage thickness of BCs.In figleaf gourd plants,CA treatment had slight effects on root length and activity,decreased number and viability of BCs and increased mucilage thickness of BCs.SNP soaking with concentrations of 50 and 100μmol/L increased number and viability of BCs.SNP soaking with concentration of 400μmol/L decreased root length,number and viability of BCs.【Conclusion】SNP presoaking at concentrations of 50 to 100μmol/L alleviated the effects of CA on cucumber and 100μmol/L was better.CA insignificantly inhibited growth of figleaf gourd,and the alleviation by SNP was less.

关 键 词：硝普钠 肉桂酸 黄瓜 黑籽南瓜 边缘细胞 黏胶层 

分 类 号：S642.101[农业科学—蔬菜学]