川东地区汉族人群18个STR基因座遗传多态性  

Genetic polymorphisms of 18 STR loci in the Han population of Eastern Sichuan

作　　者：汪昌丽[1] 范英南[1] 樊哲仁[2] 姚伊人[3] 杨帆[3] 张国栋[1] 喻永敏[1] WANG Changli;FAN Yingnan;FAN Zheren;YAO Yiren;YAN Fan;ZHANG Guodong;YU Yongmin(Institute of Forensic Science,Chongqing Municipal Public SecurityBureau,Chongqing 400707,China;Banan Branch of Chongqing Municipal Public SecurityBureau,Chongqing 401320,China;Institute of ForensicScience,Ministry of Public Security(IFSC),Beijing 100038,China)

机构地区：[1]重庆市公安局物证鉴定中心,400707 [2]重庆市公安局巴南区分局,401320 [3]公安部物证鉴定中心,北京100038

出　　处：《检验医学与临床》2019年第1期1-4,共4页Laboratory Medicine and Clinic

基　　金：公安部物证鉴定中心开放课题(2015FGKFKT02);公安部技术研究计划项目(2016JSYJC10);重庆市社会民生保障项目(cstc2015shmszx00013)

摘　　要：目的应用直接扩增法批量检验并调查川东地区汉族人群DNA标本在18个STR基因座的遗传多态性。方法采用DNATyper15TMPlus试剂盒直接扩增和3730XL型遗传分析仪检测STR分型。随机抽取326例该地区汉族无关个体STR分型结果,并统计18个STR基因座的等位基因分布频率及其他群体遗传学参数。结果 18个STR基因座基因型的分布符合Hardy-Weinberg平衡(P>0.05),川东地区汉族人群18个STR基因座的杂合度、匹配概率、多态信息含量、个体识别概率、非父排除概率的范围分别为0.604～0.896、0.016～0.219、0.545～0.908、0.781～0.984、0.295～0.788。结论使用直接扩增法批量检验DNA数据库标本的效率高、质量好;18个STR基因座的联合应用能获得较好的系统效能,为该地区汉族人群的遗传多态性研究及应用提供数据支持。Objective To investigate the genetic polymorphism of 18 STR loci by a high-throughput direct amplification method.Methods DNATyper15 TM Plus kit,ABI 9700 PCR and ABI3730XL genetic analyzer were used to test 326 STR profiles of unrelated Han individuals from Eastern Sichuan.The population genetic parameters were calculated by statistic software Powerstat.Results The results demonstrated that all 18 STR loci were in accordance with the distribution of Hardy-Weinberg equilibrium(P>0.05).Heterozygosity(H)ranged from 0.604―0.896 matching probability(PM)ranged from 0.016―0.219,power of discrimination(DP)ranged from 0.803-0.985,polymorphism information content(PIC)ranged from 0.781―0.984,power of exclusion(PE)ranged from 0.295―0.788.Conclusion 18 STR loci in Han population in Eastern Sichuan have higher polymorphisms.

关 键 词：直接扩增 STR基因座 遗传多态性 川东地区 汉族 

分 类 号：D919.2[医药卫生—法医学]