产吲哚金黄杆菌耐药性和耐药基因研究  

The study on drug resistance and resistant genotype of Chryseobacterium indologenes

作　　者：解春宝[1] 罗江蓉 赵秋敏 喻华[1] 传良敏[1] XIE Chunbao;LUO Jiangrong;ZHAO Qiumin;YU Hua;CHUAN Liangmin(Clinical Laboratory Department,Sichuan Academy of Medical Sciences & SichuanProvincial People′s Hospital,Chengdu,Sichuan 610072,China)

机构地区：[1]四川省医学科学院/四川省人民医院检验科,成都610072 [2]四川省医学科学院/四川省人民医院运营部,成都610072 [3]川北医学院,四川南充637000

出　　处：《检验医学与临床》2019年第1期20-22,26,共4页Laboratory Medicine and Clinic

基　　金：四川省卫生和计划生育委员会科研课题(16PJ470);四川省医学科学院/四川省人民医院青年基金(2015QN03);四川省医学科学院/四川省人民医院苗圃科研课题(重点项目30305030884)

摘　　要：目的本研究拟了解临床分离产吲哚金黄杆菌的耐药特性,检测其耐药基因分析耐药机制,为临床合理使用抗菌药物提供科学依据。方法收集四川省人民医院2013年10月至2015年2月临床分离的产吲哚金黄杆菌菌株,分析其耐药谱。利用聚合酶链反应(PCR)技术筛查其耐药基因,扩增阳性产物进行序列测定然后将其结果与GenBank数据库进行比对分析以确定其基因型。结果共收集到6株产吲哚金黄杆菌,6株菌株对青霉素类、头孢菌素类、碳青霉烯类等抗菌药物表现为耐药。耐药基因检测结果:5株细菌携带IND-2、IND-LIKE、CIA耐药基因;未检测到携带IND-1、IND-3、IND-4耐药基因的细菌。结论该院临床分离的产吲哚金黄杆菌表现为多重耐药,其耐药基因型以IND-2和CIA为常见。Objective To investigate the drug resistance and resistant genotype of Chryseobacterium indologenes isolated from clinical,and to provide scientific basis for clinical rational use of antibiotics.Methods Chryseobacterium indologenes strains were collected from a hospital in Sichuan Province from October 2010 to February 2015,and its drug resistance spectrum was analyzed.The drug resistance gene was screened by polymerase chain reaction(PCR).The positive amplification products were sequenced and then compared with GenBank database to determine the drug resistance genotype.Results Six strains of Chryseobacterium indologenes were collected and six strains were resistant to antimicrobial agents such as penicillins,cephalosporins and carbapenems.The results showed that IND-2,IND-LIKE and CIA resistant genotypes were positive in 5 strains.IND-1,IND-3 and IND-4 resistant genotype were negative in all the 6 strains.Conclusion At present,the Chryseobacterium indologenes isolated from the hospital showes multiple drug resistance,and their resistance genotype are common in IND-2 and CIA.

关 键 词：产吲哚金黄杆菌 耐药谱 基因型 

分 类 号：R378.99[医药卫生—病原生物学]