一锅煮法合成5-甲基四氢叶酸钙  

One-pot Synthesis of Calcium 5-Methyltetrahydrofolate

作　　者：张珍明[1] 陈达 谭超兰 占垚 李树安[1] 彭晓涵 夏天 张雪冬 ZHANG Zhenming;CHEN Da;TAN Chaolan;ZHAN Yao;LI Shu’an;PENG Xiaohan;XIA Tian;ZHANG Xuedong(Jiangsu Marine Resources Development Research Institute,Lianyungang 222005,China)

机构地区：[1]江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《淮海工学院学报（自然科学版）》2018年第4期47-51,共5页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(21606095);国家海洋公益性行业科研专项(201305007);淮海工学院博士启动基金(KQ16001);淮海工学院大学生实践创新训练计划项目(201811641319001);淮海工学院教学改革研究课题(XJG2017-3-16);连云港市工业攻关项目(CG1613);连云港市港城微.博双创计划项目(201705)

摘　　要：以叶酸为原料,采用一锅煮法合成5-甲基四氢叶酸,用活性炭吸附和半胱氨酸、乙醇和水混合液洗脱进行提纯分离,再与氯化钙反应制备较稳定的5-甲基四氢叶酸钙,最佳反应条件为n(叶酸):n(硼氢化钠)=1∶12,甲基化时间为2h,甲基化温度为30℃,活性炭吸附时间为3h,洗脱时间为5h.此条件下5-甲基四氢叶酸钙收率为82.4%,纯度为92.7%.用HPLC,元素分析,IR,UV,1 H NRM,MS等方法对目标产物结构进行了表征.5-methyltetrahydrofolic acid was synthesized from folic acid by one-pot method.It was separated and purified by adsorption with activated carbon and elution in virtue of cysteine-ethanol-water mix,then reacted with calcium chloride to obtain a more stable compound of calcium 5-methyltetrahydrofolic acid.The optimal reaction conditions were as follows:the molar ratio of folic acid and borohydride was 1∶12,methylation reaction time was 2 h,methylation reaction temperature was 30℃,the adsorption time of activated carbon is 3 hand the elution time is 5 h.The yield of calcium 5-methyltetrahydrofolate is 82.4%,with the purity of 92.7%.Its structure was characterized by HPLC,elemental analysis,IR,UV,1 H NRM,MS.

关 键 词：一锅煮法 合成 5-甲基四氢叶酸钙 

分 类 号：TQ463[化学工程—制药化工] O626[理学—有机化学]