辣椒疫霉病生防细菌的筛选鉴定及其防效  

Screening,Identification and Control Effect of a Bio-control Bacteria for Pepper Phytophthora Blight

作　　者：赵旖森 张亮[1] 盛浩[1] 袁红[1] 周清[1] ZHAO Yi-sen;ZHANG Liang;SHENG Hao;YUAN Hong;ZHOU Qing(College of Resource and Environment,Hunan Agricultural University,Changsha 410128,Hunan,China)

机构地区：[1]湖南农业大学资源环境学院,湖南长沙410128

出　　处：《中国蔬菜》2019年第1期65-69,共5页China Vegetables

基　　金：国家自然科学基金项目(41571234);湖南农业大学"1515"学术创新团队带头人培养项目;湖南农业大学青年科学基金项目;湖南农业大学大学生科技创新项目(2017ZK36)

摘　　要：以筛选、获取优良辣椒疫霉病害生防菌株材料为目标,通过平板稀释法与对峙法对水稻土壤耕层可培养细菌进行分离和筛选,成功从167株可培养细菌中分离、筛选获得1株拮抗菌LRS-1,并被鉴定为多粘类芽孢杆菌(Paenibacillus polymyxa)。该拮抗菌对辣椒疫霉菌具有68%左右的室内持久抑菌率以及63.4%的温室病害防效;Realtime-PCR检测结果表明该拮抗菌对根围疫霉菌侵染数量具有显著的抑制效果。In order to screen and obtain good bio-control bacterial strain,this study isolated and screened cultivable bacteria from the top paddy soil by means of dilution method and inhibition test in plate.An effective antagonistic bacteria named LRS-1 was isolated from 167 cultivable bacteria strains.It was identified as Paenibacillus polymyxa bacteria.The strain owned as much as 68%inhibition rate against Phytophthora capsici in door and 63.4%controlling effect on Phytophthora blight of pepper in greenhouse.The result of Real-time PCR test indicated that this strain had remarkable inhibitory effect on Phytophthora pathogen population in rhizosphere.

关 键 词：辣椒疫霉 拮抗菌 筛选 鉴定 防效 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]