3种微藻生物量监测方法的比较  

An Optimal Method for Biomass Measurement of Microalgae

作　　者：刘积光 李昂[2] 刘平怀[2] LIU Jiguang;LI Ang;LIU Pinghuai(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China;Department of Materials and Chemical Engineering,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带农林学院,海口570228 [2]海南大学材料与化工学院,海口570228

出　　处：《热带生物学报》2018年第4期457-463,共7页Journal of Tropical Biology

基　　金：国家科技型中小企业技术创新基金项目(13C26244604892);海南省产学研一体化项目(CXY20150034);海南省中药现代化科技专项(ZY201327);国家科技支撑计划项目(2011BAD14B01);海口市应用技术研究与开发项目(2017044)

摘　　要：对生物质干重法、细胞计数法和光密度法3种微藻生物量监测方法进行比较研究,以BG11为基础培养基,在光自养分批培养模式下完成对热带海洋富油微藻Desmodesmus sp. WC08整个生长周期内的生物量变化监测,结合梯度稀释法,对不同浓度藻液的光密度(OD)、细胞密度和生物质干质量进行测定,建立3种测定微藻生物量方法的数学联系。在最大吸收波长680 nm下,对数生长期的微藻细胞密度与OD值存在线性关系,即,y=0. 336 66x-0. 002 88,R2=0. 998 7;生物质干质量与OD值呈线性关系,直线方程为:y=1. 095 98x-0. 064 04,R2=0. 973 14。分别考察了5种不同培养基下,Desmodesmus sp. WC08生长过程中光密度与细胞密度监测方法结果的差异,以及同一培养基培养5株不同微藻,在生长过程中光密度与细胞密度监测结果的差异。比较结果表明,通过测定微藻的光密度(OD)能较准确的计算出藻液的生物量、生物质干质量和细胞密度。光密度法简便可靠,更适用于微藻培养过程中的生物量监测。Five microalgae species,i.e.Desmodesmus sp.WC08,Chlorella sp.C74,Monoraphidium sp.C29,Micractinium sp.C67 and Chlorella sp.QH were batch cultured under photo-autotrophy with blue-green(BG-11)medium as a basic medium,and their biomasses were measured during their growth.The microalgae cultures were gradient diluted to determine their optical density(OD),cell density(CD)and dry weight(DW),with which mathematical relations with the biomass of the microalgae were established.At the maximum absorption wavelength of 680 nm,the CD and the OD of the microalgae at the exponential growth stage had a linear relation,which was,expressed as y=0.336 66x-0.002 88,R^2=0.998 7;the DW was linearly related with the OD in a linear equation of y=1.095 98 x-0.064 04,R^2=0.973 14.The difference between the OD and the CD using Desmodesmus sp.WC08 on five different mediums and the difference of five different microalgae species cultured on the same medium between the OD and the CD were compared and analyze.The results showed that the OD was the best index for calculating of biomass,dry weight and cell density of the microalgae.The OD determination is hence the best method for biomass measurement of the microalgae during the culture.

关 键 词：生长监测 光密度 细胞计数 干质量 

分 类 号：Q93-332[生物学—微生物学]