利用荧光定量PCR和荧光定量RT-PCR检测猪瘟活疫苗(细胞源)中的16种外源病毒  

Detection of 16 Exogenous Viruses from Living Classical Swine Fever Vaccine by Fluorescence Quantitative PCR and Fluorescence Quantitative RT-PCR

作　　者：孙博 宋新刚 张萌萌 张健 高习文 张立恒 尹辉 郭伟 任德强 Sun Bo;Song Xin’gang;Zhang Mengmeng;Zhang Jian;Gao Xiwen;Zhang Liheng;Yin Hui;Guo Wei;Ren Deqiang(Harbin Yuanheng Biological Pharmaceutical Co.Ltd.Harbin,150025,China)

机构地区：[1]哈尔滨元亨生物药业有限公司,黑龙江哈尔滨150025

出　　处：《现代畜牧科技》2019年第1期1-4,共4页Modern Animal Husbandry Science & Technology

摘　　要：本文为分子生物学方法检测猪瘟活疫苗(细胞源)外源病毒提供实验数据。用经过验证的荧光定量PCR和荧光定量RT-PCR方法检测哈尔滨元亨生物药业有限公司生产的24批猪瘟活疫苗(细胞源)中的16种外源病毒(FMDV、PCV2、CSFVW、BCoV、BVDV、BPIV-3、TGEV、PEDV、RV、PRRSV、PCV1、MRV、JEV、PPV、PRV、BHV-1),结果显示24批猪瘟活疫苗(细胞源)成品均无以上16种外源病毒。It provides experimental data for the determination of classical live swine fever vaccine(cell source)exogenous virus by molecular biology method.Detection of 16 exogenous viruses(FMDV、PCV2、CSFVW、BCoV、BVDV、BPIV-3、TGEV、PEDV、RV、PRRSV、PCV1、MRV、JEV、PPV、PRV、BHV-1)from 24 batches of live classical swine fever vaccine(cell source)produced by Harbin Yuanheng Biopharmaceuticals Co.Ltd.,the results showed that 24 batches of products did not have the above 16 exogenous viruses.

关 键 词：猪瘟活疫苗 PCR RT-PCR 外源病毒 

分 类 号：S852.4[农业科学—基础兽医学]