籼稻品种‘BP360e’抗褐飞虱遗传分析及基因定位  

Genetic analysis and mapping of the gene conferring brown planthopper resistance in indica rice cultivar 'BP360e'

作　　者：陶蓉 潘根 江玲[1] 程遐年[1] 刘裕强[1] 万建民[1,2] TAO Rong;PAN Gen;JIANG Ling;CHENG Xianian;LIU Yuqiang;WAN Jianmin(State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University, Nanjing 210095,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《南京农业大学学报》2019年第1期14-20,共7页Journal of Nanjing Agricultural University

基　　金：国家重点研发计划项目(2016YFD0100603-9);国家自然科学基金项目(31522039;31471470);江苏省自然科学基金项目(BK20150026)

摘　　要：[目的]本研究旨在发掘水稻抗褐飞虱基因,为抗褐飞虱品种培育提供有用的基因资源。[方法]利用印尼抗褐飞虱籼稻品种‘BP360e’与感虫品种‘02428’分别构建F2∶3和BC2F2∶3分离群体,采用苗期集团法进行抗褐飞虱表型鉴定。利用151对均匀分布在水稻12条染色体的多态性标记,选取F2∶3群体中的极端个体进行初连锁分析,构建具有连锁迹象的染色体连锁图谱进行QTL(quantitative trait loci)检测。再利用BC2F2∶3群体验证F2∶3群体检测到QTL的可靠性。[结果]在第4染色体标记RM16382与INDEL4-5之间,约15.3 c M的区间内检测到1个抗褐飞虱主效QTL,LOD值为14.4,贡献率为56%,将其命名为QBph4。用BC2F2∶3群体重复检测到该QTL。该区间包含已克隆的抗褐飞虱基因Bph3。序列分析表明,QBph4可能为Bph3的一种新的等位基因。[结论]‘BP360e’高抗褐飞虱,其抗性受1对主效QTL控制,该抗性基因的发掘为水稻抗褐飞虱分子机制阐明及新品种培育奠定了基础。[Objectives] The aim of present study is to explore novel BPH resistance gene for breeding BPH resistance rice cultivars.[Methods] An F2∶3 and a BC2F2∶3 populations were constructed by crossing resistant indica cultivar‘BP360e’with susceptible japonica cultivar‘02428’,respectively,and then evaluated for BPH resistance by standard seed box screening test. Further,151 polymorphic SSR markers between the parents uniformly distributed on 12 chromosomes of rice,extreme resistant and susceptible lines selected from F2∶3 population were used primarily for linkage analysis,and the molecular linkage map of candidate chromosome was applied to detect the QTL conferring BPH resistance. Then,the QTL was verified by BC2F2∶3 population.[Results] A major QTL on chromosome 4 was detected in the 15.3cM interval of RM16382 and INDEL4-5,with LOD score of 14.4,explained the phenotypic variance of 56%,and subsequently designated as QBph 4 . Sequencing analysis showed that QBph4 probably was a novel allele for Bph3 ,a previously cloned BPH resistance gene.[Conclusions] The indica cultivar‘BP360e’had a highly resistance against BPH,which was contributed by a major QTL. The discovery of this resistance gene has laid a foundation for elucidating the underling mechanism of BPH resistance and breeding resistance rice cultivars.

关 键 词：水稻 褐飞虱 抗性 数量性状基因座 

分 类 号：S511.032[农业科学—作物学]