胡杨树脂提取物抗炎活性筛选及化学成分分析  

Anti-inflammatory Activity and Chemical Composition of Resin Extracts from Populus diversifolia Schrenk.

作　　者：龚雪 李柱 张娜[1] 王杰 韩奇亨 任凯[1] 张春红[1,3,4] 李旻辉 GONG Xue;LI Zhu;ZHANG Na;WANG Jie;HAN Qi-heng;REN Kai;ZHANG Chun-hong;LI Min-hui(Baotou Medical College,Baotou 014060,China;Ejin Banner Mongolian medicine Hospital,Ejin Banner 735400,China;Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology,Baotou 014060,China;Inner Mongolia Key Laboratory of Characteristic Traditional Chinese Medicinal Resources Protection and utilize,Baotou 014060,China;Inner Mongolia Autonmous Region Academy of Chinese Medicine,Hohhot 010110,China;Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China)

机构地区：[1]包头医学院,内蒙古包头014060 [2]额济纳旗蒙医医院,内蒙古阿拉善盟735400 [3]内蒙古自治区特色药用植物培育与保护工程技术研究中心,内蒙古包头014060 [4]内蒙古自治区特色道地药材资源保护与利用重点实验室,内蒙古包头014060 [5]内蒙古自治区中医药研究所,内蒙古呼和浩特010110 [6]广西药用植物园/广西药用资源保护与遗传改良重点实验室,广西南宁530023

出　　处：《中国现代中药》2018年第12期1494-1498,共5页Modern Chinese Medicine

基　　金：阿拉善盟2013年应用技术研究与开发资金项目(2013-36);包头医学院博士科研启动基金项目(BSJJ201610)

摘　　要：目的:探讨胡杨树脂提取物对脂多糖(LPS)刺激小鼠单核巨噬白血病细胞RAW 264. 7细胞炎症保护作用及利用UPLC-Q-Exactive四级杆-静电场轨道阱高分辨质谱联用技术,对胡杨树脂化学成分进行识别和鉴定。方法:用不同浓度(12. 5、25、50、100、200μg·m L-1)的胡杨树脂提取物处理RAW 264. 7细胞,MTT法检测细胞增殖活性;通过LPS(1μg·m L-1)诱导RAW 264. 7细胞建立炎症模型,用不同浓度胡杨树脂提取物处理炎症模型,MTT法检测胡杨树脂提取物对细胞的保护作用;采用UPLC-Q-Exactive四级杆-静电场轨道阱高分辨质谱仪联用技术,借助数据挖掘方法识别和鉴定胡杨树脂中的化学成分,对其高分辨一级和二级质谱数据进行解析,并与数据库比对,对胡杨树脂化学成分的结构推测和确认。结果:在实验浓度范围内,胡杨树脂提取物对细胞无细胞毒性作用;与LPS模型组比较,浓度为12. 5、25、50、100、200μg·m L-1胡杨树脂提取物均能显著改善LPS诱导小鼠巨噬细胞RAW 264. 7的炎症反应,具有较好的浓度依赖性;通过高分辨数据推测鉴定出6个化合物,分别为棕榈油酸、邻苯二甲酸二丁酯、十六酰胺、油酸酰胺、硬脂酰胺、芥酸酰胺。结论:该结果为胡杨树脂的提取分离及抗炎活性作用研究奠定基础,为进一步对胡杨树脂的综合研究提供科学的依据。Objective:To investigate the protective effects of resin extracts from Populus diversifolia on the inflammation of mice mononuclear macrophage RAW 264.7 cells induced by lipopolysaccharide(LPS).An ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-hybrid quadrupole-orbitrap MS)method was developed toanalyze and identify the chemical constituents from resin of P.diversifolia.Methods:The RAW 264.7 cells were treated with different concentrations(12.5,25,50,100,200μg·mL^-1)of resin extracts from P.diversifolia,and cell viability was determined by MTT method.The infla mmation model which was established by LPS(1μg·mL^-1)-induced RAW 264.7 cells were used to determine the resin extracts from P.diversifolia.The compounds were found and identified through the serious data mining,structural speculation on basis of the careful analysis of the MS and MS/MS data,and confirmed by comparison with the references and library.Results:The resin extracts from P.diversifolia had no cytotoxicity to RAW 264.7 cells in the experimental concentration range.Compared with model group,the resin extracts from P.diversifolia at the concentrations of 12.5,25,50,100,200μg·mL^-1 could significantly improve the inflammation of RAW 264.7 cells induced by LPS,and showed a well dose-dependent manner.A total of 6 compounds,including palmitoleic acid,dibutyl phthalate,hexadecanamide,oleamide,octadecanamide and erucylamide were identified.Conclusion:The results provide scientific evidence for further studying its chemical composition and pharmacological activity of the P.diversifolia comprehensively and objectively.

关 键 词：胡杨树脂 RAW264.7细胞 抗炎 UPLC-Q-Exactive四级杆-静电场轨道阱高分辨质谱 

分 类 号：R284[医药卫生—中药学]