止泻木子中锥丝碱的提取工艺  

Study on Extraction Technology of Conessine from Holarrhena antidysenteriaca

作　　者：郑光杰 邱娜 刘凡凡[2] 晋敦青 孙博航 ZHENG Guang-jie;QIU Na;LIU Fan-fan;JIN Dun-qing;SUN Bo-hang(School of Key Laboratory of Structure Based Drug Design & Discovery of Ministry of Education,School of Tradition Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;Zhengzhou Central Hospital Pharmacy Department,Zhengzhou 467000,China)

机构地区：[1]沈阳药科大学中药学院/基于靶点的药物设计与研究教育部重点实验室,辽宁沈阳110016 [2]郑州市中心医院药学部,河南郑州467000

出　　处：《中国现代中药》2018年第12期1544-1548,共5页Modern Chinese Medicine

基　　金：辽宁省教育厅科学技术研究项目(201610163120)

摘　　要：目的:优化止泻木子中锥丝碱的提取工艺,为止泻木子质量控制标准的建立奠定基础。方法:在单因素试验基础上,以提取时间、甲醇浓度、料液比为因素,以止泻木子中锥丝碱提取得率为响应值,采用三因素三水平响应曲面试验设计法,确定最佳提取工艺。结果:最佳提取工艺为提取时间70 min、甲醇浓度75%、料液比1∶9。结论:该提取工艺稳定、合理、预测性良好。Objective: The process of extracting conessine from Holarrhena antidysenteriaca optimized for establishing the quality control standards of Holarrhena antidysenteriaca. Methods: Based on the results of single factor tests,we selected extraction time,methanol concentration and solid/liquid ratio as the experimental factors and used the extraction yield of conessine from as the response value. Results: The optimal extraction parameters were as follows: extraction time 70 min,methanol concentration 75%,solid/liquid ratio 1: 9. Conclusion: The extraction method is reliable,reasonable and highly predictable.

关 键 词：止泻木子 锥丝碱 响应曲面 提取工艺 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]