基于纳米探针和石墨烯电极技术的ATP生物发光检测系统  

A Fast Detecting System Based on Nanoprobe and Graphite Electrode with ATP Bioluminescence Technology

作　　者：徐元达 王春兴[1] XU Yuan-da;WANG Chun-xing(School of Physics and Electronic,Shandong Normal University,250014,Jinan,Shandong,PRC)

机构地区：[1]山东师范大学物理与电子科学学院,山东省济南市250014

出　　处：《曲阜师范大学学报（自然科学版）》2019年第1期62-68,共7页Journal of Qufu Normal University(Natural Science)

基　　金：国家自然科学基金(11674198)

摘　　要：由细菌引起的食品安全问题越来越受到人们的重视,使得快速细菌检测技术成为一个重要课题.设计了一种基于纳米探针技术、石墨烯透明电极(GTE)技术和三磷酸腺苷(ATP)发光技术的生物检测系统,能够快速准确地检测食品中的细菌.实验结果表明该方法与传统检测方法具有很好的相关性,线性相关度可达0.972,检测时间控制在20min内.检测范围在102-106 CFU/mL.此外通过检测不同污染物测得该系统具有令人满意的性能.可以满足现场快速检测的要求.Food safety issues caused by the bacteria have drawn people′s attention increasingly,fast and accurate detection technology becomes an urgent research topic.The biological detection system based on nano-probe technology,graphene transparent electrode(GTE)technology and adenosine triphosphate(ATP)luminescence technology is designed in this paper,it can detect the bacteria of food automatically and rapidly.The results indicate that there is a good correlation between the rapid detecting method and the other conventional approaches.the linear coefficient can be up to 0.972,and the detecting time can be controlled within twenty minutes.The detectable bacteria concentration range reaches 10^2-10^6 CFU/mL.Moreover,a series of curves obtained by measurements for different polluted foods show that the system has a satisfactory performance.It is sufficient to meet the requirements of on-site rapid detection.

关 键 词：纳米磁球 石墨烯透明电极 ATP荧光 快速检测 

分 类 号：TN386.6[电子电信—物理电子学]