2种炮制方法对何首乌中6种成分变化的影响  

Effects of two processing methods on the changes of six constituents in Polygonum multiflorum

作　　者：杨磊[1,2] 刘梦娇 陈文明 戴冰[1] 张志国 张琴[1,2] YANG Lei;LIU Meng-jiao;CHEN Wen-ming;DAI Bing;ZHANG Zhi-guo;ZHANG Qin(The First Hospital Affiliated to Hunan University of Chinese Medicine,Changsha 410007,China;ZHANG Zhi-guo National Heritage of Traditional Chinese Medicine Expert Studio,Changsha 410007,China;Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]全国名老中医药专家张志国传承工作室,湖南长沙410007 [3]湖南中医药大学,湖南长沙410208

出　　处：《中成药》2019年第1期119-123,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学青年基金(81804075);湖南省自然科学基金(2017JJ3247);湖南省发改委基金([2016] 518)

摘　　要：目的探索"九蒸九晒"、药典法2种炮制方法对何首乌中6种成分含有量变化的影响。方法何首乌甲醇提取液的分析采用Agilent C_(18)色谱柱(250 mm×4. 6 mm,5μm);流动相乙腈-0. 01%磷酸,梯度洗脱;体积流量1. 0 mL/min;检测波长295 nm;柱温30℃。结果 6种成分在各自范围内线性关系良好(R^2>0. 995 0),平均加样回收率98. 96%～101. 23%,RSD 0. 41%～1. 37%。结论何首乌在2种炮制方法下6种成分含有量存在较大差异,这些差异是否与疗效和肝毒性相关还有待进一步探讨。AIM To explore the effects of two processing methods(“nine steamed and nine shined”method and pharmacopoeia method) on the content changes of six constituents in Polygonum multiflorum Thunb.. METHODS The analysis of methanol extract of P. multiflorum was performed on a 30 ℃ thermostatic Agilent C18 column (250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0. 01% phosphoric acid flowing at 1. 0 mL /min in a gradient elution manner,and the detection wavelength was set at 295 nm. RESULTS Six constituents showed good linear relationships within their own ranges ( R^2 >0. 995 0),whose average recoveries were 98. 96%- 101. 23% with the RSDs of 0. 41%- 1. 37%. CONCLUSION There are great differences in the contents of six constituents in P. multiflorum between two processing methods,but whether these differences are related to efficacy and hepatotoxicity remains to be further explored.

关 键 词：何首乌 化学成分 “九蒸九晒” 药典法 

分 类 号：R284.1[医药卫生—中药学]