甘草微型饮片切制工艺及UPLC指纹图谱  

Cutting process and UPLC fingerprints of Glycyrrhiza uralensis mini slices

作　　者：任晓航 岳英男 魏晓峰 李祥溦 刘博男 史辑[1,2,3] REN Xiao-hang;YUE Ying-nan;WEI Xiao-feng;LI Xiang-wei;LIU Bo-nan;SHI Ji(School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;State Administration of Traditional Chinese Medicine Key Laboratory of Analysis of Processing Principle, Dalian 116600, China;Liaoning Research Center of Traditional Chinese Medicine Processing Engineering, Dalian 116600, China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]国家中医药管理局炮制原理解析重点实验室,辽宁大连116600 [3]辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中成药》2019年第1期124-129,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81473350);辽宁省自然科学基金(201602490)

摘　　要：目的优化甘草微型饮片切制工艺,建立其UPLC指纹图谱。方法以流动性、出膏率以及甘草苷、甘草酸、甘草素、甘草次酸、异甘草素、查耳酮含有量为评价指标,软化时间、饮片厚度、饮片粒径、干燥温度为影响因素,正交试验优化切制工艺。甘草70%乙醇提取物的分析采用BEH C_(18)色谱柱(2. 1 mm×50 mm,1. 7μm);流动相乙腈-0. 1%磷酸,梯度洗脱;体积流量0. 3 mL/min;柱温25℃;检测波长237 nm。结果最佳工艺为粒径0. 5～0. 9 mm,厚度1 mm,软化时间1 h,干燥温度50℃。10批样品UPLC指纹图谱中有25个共有峰,相似度均大于0. 9。6种成分在各自范围内线性关系良好(R^2>0. 999 0),平均加样回收率为99. 4%～102. 5%,RSD 0. 29%～1. 9%。结论该方法准确稳定,重复性好,可用于甘草微型饮片的质量控制。AIM To optimize the cutting process and to establish UPLC fingerprints of Glycyrrhiza uralensis Fisch mini slices. METHODS With flowing time, leaching rate and the contents of glycyrrhizin, glycyrrhizin, glycyrrhizin, glycyrrhetinic acid, isoliquiritigenin, chalcone as evaluation indices, soften time, pieces thickness, pieces diameter and drying temperature as influencing factors, the cutting process was optimized by orthogonal test. The analysis of 70% ethanol extract of G. uralensis was performed on a 25 ℃ thermostatic BEH C18 column (2.1 mm×50 mm, 1.7 μm), with the mobile phase comprising of acetonitrile?0.1% phosphoric acid flowing at 0.3 mL/ min in a gradient elution manner, and the detection wavelength was set at 237 nm. RESULTS The optimal cutting process conditions were determined to be 0.5-0.9 mm for the grain size, 1 mm for the thickness, 1 h for the soften time, and 50 ℃ for the drying temperature. There were twenty?five common peaks in the UPLC finge-prints of ten batches of samples with the similarities of more than 0.9. Six constituents showed good linear relation-ships within their own ranges (R^2 >0.9990), whose average recoveries were 99.4%-102.5% with the RSDs of 0.29%-1.9%. CONCLUSION This accurate, stable and reproducible method can be used for the quality con-trol of G.uralensis mini slices.

关 键 词：甘草 微型饮片 切制工艺 正交试验 UPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]