心脑舒通胶囊对氧糖剥夺/复氧星形胶质细胞神经营养因子分泌的影响及对神经元的保护作用  

Effect of Xinnao Shutong Capsules on secretion of neurotrophic factor in oxygen-glucose deprivation/reoxyge nation astrocytes and protection of neurons

作　　者：李芮琳 刘文杰 袁庆[1] 张彤 徐耀 胡利民 柴丽娟 LI Ruilin;LIU Wenjie;YUAN Qing;ZHANG Tong;XU Yao;HU Limin;CHAI Lijuan(Institute of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae Ministry of Education,Tianjin 300193,China;Tianjin Key Laboratory of Chinese Medicine Pharmacology,Tianjin 300193,China)

机构地区：[1]天津中医药大学中医药研究院,天津300193 [2]方剂学教育部重点实验室,天津300193 [3]天津市中药药理学重点实验室,天津300193

出　　处：《天津中医药》2019年第1期63-66,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81573644);国家重大新药创制项目(2011ZX09201-201-33);"十三五"期间天津市高等学校创新团队

摘　　要：[目的]探讨心脑舒通胶囊对氧糖剥夺/复氧(OGD/R)处理的星形胶质细胞(C8-D1A)神经生长因子分泌的影响,以及研究心脑舒通药物处理的OGD/R胶质细胞条件培养液对神经元细胞(Neuro-2A)的保护作用。[方法] CCK-8检测心脑舒通对正常培养和OGD/R胶质细胞活力的影响;酶联免疫吸附(ELISA)法检测心脑舒通对OGD/R胶质细胞的脑源性神经营养因子(BDNF)、胶质源性神经营养因子(GDNF)、睫状神经营养因子(CNTF)释放的影响;CCK-8检测心脑舒通处理的胶质细胞条件培养液对OGD/R神经元细胞活力的影响。[结果] 1)心脑舒通对正常培养的胶质细胞活力无显著影响,但可以提高OGD/R后胶质细胞的增殖活力。2)0.1μg/mL心脑舒通可以显著提高OGD/R胶质细胞BDNF的释放。3)0.1μg/mL心脑舒通药物处理的胶质细胞条件培养液可提高OGD/R神经元的存活能力。[结论]心脑舒通对OGD/R损伤后的胶质细胞有一定保护作用,其处理后的胶质细胞条件培养液对神经元有一定的保护作用。[Objective] To explore the effect of Xinnao Shutong Capsules on the secretion of nerve growth factor in astrocytes cell induced by oxygen-glucose deprivation/reoxygenation (OGD/R).The protective effects on OGD/R-induced neurons cell variability treated by astrocytes conditioned medium were also explored.[Methods] The effect of Xinnao Shutong Capsules on both normal culture and OGD/R-induced C8-D1A cell viability were detected by CCK-8.ELISA was adopted to measure the secretion of BDNF,GDNF and CNTF in OGD/R-induced C8-D1A cell which treatment with Xinnao Shutong Capsules.[Results] 1)Xinnao Shutong Capsules had no significant effect on the viability of normal cultured C8-D1A cell,but could increase the proliferation of OGD/R-induced C8-D1A cell.2)Xinnao Shutong Capsules (0.1 μg/mL )could significantly promote the secretion of BDNF on C8-D1A cell after OGD/R.3)C8-D1A cell treated with Xinnao Shutong Capsules (0.1 μg/mL)conditioned medium had protective effect on neurons.[Conclusion] Xinnao Shutong Capsules had protective effect on OGD/R-induced C8-D1A cell,and its treated C8-D1A conditioned medium played protective role on OGD/R-induced neurons.

关 键 词：心脑舒通胶囊 星形胶质细胞 神经元 脑源性神经营养因子 胶质源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]