氯冉酸荷移分光光度法测定注射用头孢硫脒  

Spectrophotometry deter mination of Cafathiamidine for injection based on charge transfer complex of Cafathiamidine with chloranilic acid

作　　者：张卫[1] 刘昌发[1] 陈萍萍 马润涛 李健[1] Zhang Wei;Liu Changfa;Chen Pingping;Ma Runtao;Li Jian(Department of pharmacy,Zhengzhou Railway Vocational and Technical College,Zhengzhou 451460)

机构地区：[1]郑州铁路职业技术学院药学系,郑州451460

出　　处：《北方药学》2019年第2期12-13,共2页Journal of North Pharmacy

摘　　要：目的:建立测定注射用头孢硫脒含量的方法。方法:利用氯冉酸与头孢硫脒在无水乙醇溶液中发生荷移反应,形成荷移络合物,采用分光光度法测定。结果:荷移络合物在530nm处有最大吸收,表观摩尔吸收系数为1.164×103L/(mol·cm),药物浓度在0.007840～0.1702mg/mL内符合朗伯比尔定律。方法的平均回收率在98.0%以上。结论:该方法快速、准确,可用于测定药物制剂中头孢硫脒的含量。Objective:To establish a method for rapid determination of Cafathiamidine for injection.Methods:To utilize visible spectrophotometry based on the charge transfer reaction between Cafathiamidine as donor and Chloranilic acid as acceptor in alcohol solution.Results:The apparent molar absorptivity of complex was 1.164×103L/(mol·cm)at 530 nm.Lambert-Beer law was obeyed in the range of 0.007840~0.1702mg/mL of Cafathiamidine.The average recovery was over 98.0%.Conclusion:The proposed method is stable,rapid and accurate.It has been applied to the determination of Cafathiamidine in preparation with satisfactory results.

关 键 词：荷移分光光度法 氯冉酸 注射用头孢硫脒 

分 类 号：R917[医药卫生—药物分析学]