二氢杨梅素对类风湿性关节炎大鼠脾脏免疫功能的调节作用  

Regulation of dihydromyricetin on immune function of spleen in the rats with rheumatoid arthritis

作　　者：吴菁[1] 范凯健 王婷玉[1] WU Jing;FAN Kai-Jian;WANG Ting-Yu(Department of Pharmacy,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Department of Pharmacy,Shanghai Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai 201901,China)

机构地区：[1]上海交通大学医学院附属第九人民医院药剂科,上海200011 [2]上海市宝山区中西医结合医院药剂科,上海201901

出　　处：《上海交通大学学报（医学版）》2018年第12期1401-1407,共7页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81301531;81572104;81874011);上海市科学技术委员会科研项目(18140903502)~~

摘　　要：目的·观察二氢杨梅素(dihydromyricetin,DMY)对类风湿性关节炎模型大鼠脾脏免疫功能的调节作用。方法·将30只Wistar大鼠随机分为5组,分别为正常组、模型组和DMY给药组(给药剂量分别为5、25和50 mg/kg),模型组和DMY给药组建立Ⅱ型胶原诱导的类风湿性关节炎(typeⅡcollagen-induced rheumatoid arthritis,CIA)大鼠模型;DMY给药组按设定剂量腹腔注射DMY,每2日注射1次。给药5周后,处死各组大鼠,取出脾脏,制作组织切片,苏木精-伊红染色后观察病理变化。分离模型组脾淋巴细胞,CCK8试剂盒检测DMY在体外对炎症状态下脾淋巴细胞增殖的影响;流式细胞术检测T淋巴细胞不同亚群的百分含量;PCR和ELISA测定脾淋巴细胞相关炎症因子的表达情况。结果·与模型组相比,DMY给药组脾脏的炎症相关病理变化明显缓解。DMY在体外可抑制伴刀豆球蛋白(ConA)诱导的脾淋巴细胞增殖,减少ConA引起的CD3^+CD4^+细胞百分比增加,高浓度时可略增加CD3^+CD8^+细胞百分比。PCR和ELISA结果表明,DMY在体内外条件下均可抑制脾淋巴细胞促炎因子的表达,同时促进抗炎因子的表达。结论·DMY在体内和体外均对CIA模型大鼠脾脏中免疫相关T淋巴细胞具有免疫调节作用,从而抑制全身性炎症反应。Objective·To investigate the immunoregulatory effect of dihydromyricetin(DMY)on the spleens of rats with rheumatoid arthritis.Methods·Thirty Wistar rats were randomly divided into five groups,i.e.,normal group,model group and DMY administration groups(5,25,and 50 mg/kg).The typeⅡcollagen-induced rheumatoid arthritis(CIA)model was established in model group and DMY administration groups.DMY administration groups were intraperitoneally injected with DMY every other day at set doses.After five weeks of administration,rats of each group were sacrificed,and the spleens were taken out to prepare tissue sections,and pathological changes were observed after hematoxylin-eosin staining.The spleen lymphocytes of model group were isolated,and the effect of DMY on the proliferation of spleen lymphocytes in inflammatory state was detected by cell-counting-kit 8.The percentages of different subsets of T lymphocytes were detected by flow cytometry.The expression of inflammatory factors in spleen lymphocytes was determined by PCR and ELISA.Results·Compared with model group,inflammation-related pathological changes in the spleens of DMY-administered groups were significantly relieved.DMY could inhibit the proliferation of spleen lymphocytes,reduce the percentage of CD3^+CD4^+cells and slightly increase the percentage of CD3^+CD8^+cells after the treatment of concanavalin(ConA)in vitro.The results of PCR and ELISA showed that DMY could inhibit the expression of proinflammatory cytokines and promote the expression of anti-inflammatory factors both in vitro and in vivo.Conclusion·DMY has immunomodulatory effects on immune-associated T lymphocytes in the spleens of CIA rats in vivo and in vitro,thereby inhibiting systemic inflammatory response

关 键 词：类风湿性关节炎 二氢杨梅素 脾脏 T淋巴细胞 大鼠 

分 类 号：R967[医药卫生—药理学]