两份玉米CMS-C恢复系的育性恢复力测定及恢复基因的分子标记定位  

Identification of fertility restoration and molecular mapping of restorer genes in two maize restore lines of CMS-C

作　　者：牟碧涛 赵卓凡 岳灵 李川 张钧 李章波 申汉 曹墨菊[1] MOU Bi-Tao;ZHAO Zhuo-Fan;YUE Ling;LI Chuan;ZHANG Jun;LI Zhang-Bo;SHEN Han;CAO Mo-Ju(Maize Research Institute,Sichuan Agricultural University/Key Laboratory of Maize Biology and Genetics and Breeding of Southwest China,Ministry of Agriculture,Chengdu 611130,Sichuan,China;Edible Fungus Sericulture Research Institute,Yibin Academy of Agricultural Sciences,Yibin 644000,Sichuan,China;Inner Mongolia Zhenjin Seed S&T Co.,Ltd,Ordos 014300,Inner Mongolia,China)

机构地区：[1]四川农业大学玉米研究所/农业部西南玉米生物学与遗传育种重点实验室,四川成都611130 [2]宜宾市农业科学院食用菌蚕桑研究所,四川宜宾644000 [3]内蒙古真金种业科技有限公司,内蒙古鄂尔多斯014300

出　　处：《作物学报》2019年第2期225-234,共10页Acta Agronomica Sinica

基　　金："十三五"国家重点研发计划项目(2016YFD0101206)资助~~

摘　　要：为了发掘更多玉米C型不育胞质的强恢复系资源,本研究对2份自交系Z16和7250-14-1进行了恢复能力的测定、恢复基因的遗传分析及恢复基因的分子标记定位。结果表明, Z16和7250-14-1对C黄早四、C478、C698-3和CMo17均表现为育性恢复,而对C48-2则均表现为育性部分恢复。通过对玉米CMS-C不同亚组胞质测交鉴定发现, Z16对G48-2、EC48-2、ES48-2、RB48-2及类48-2均表现为不育性保持,而7250-14-1对G48-2、EC48-2、ES48-2表现为育性部分恢复,对RB48-2和类48-2则表现为不育性保持。Z16和7250-14-1对CMS-T不育系均表现为不育性保持,而对CMS-S不育系则均表现为育性部分恢复。遗传分析显示, Z16对C478和C黄早四的育性恢复均受1对基因控制;而7250-14-1对C黄早四及C478的育性恢复分别受1对基因及2对基因控制。利用(C黄早四×Z16)F2、(C黄早四×7250-14-1)F2群体分别对恢复基因进行分子标记定位,其中Z16的恢复基因被定位于标记B-1至第8染色体短臂末端区域,物理距离为494 kb; 7250-14-1的恢复基因被定位于第8染色体短臂的标记B-1和Chr8-86080之间,物理距离为249kb。该研究不仅为玉米CMS-C"三系"配套的生产利用提供了恢复基因资源,也为玉米CMS-C恢复基因的克隆及恢复机制的研究奠定了一定基础。The objective of the present study was to identify novel and powerful restorer lines for CMS-C. So maize inbred lines Z16 and 7250-14-1 were crossed with both isonuclear alloplasmic and isoplasmic allonuclear CMS-C, CMS-T, and CMS-S male sterile lines. Self-cross and back-cross were conducted for some of the fertility restored F1 for genetic analysis and restorer gene mapping. Male fertility expression was investigated for all the F1, F2 and backcross populations, showing that Z16 and 7250-14-1 could restore the fertility for C Huangzaosi, C478, C698-3, and CMo17 completely, and partly restore the fertility for C48-2. Z16 could not restore the fertility for G48-2, EC48-2, ES48-2, RB48-2, and Lei48-2, while 7250-14-1 could partly restore the fertility for G48-2, EC48-2, and ES48-2, and maintain the sterility for RB48-2 and Lei48-2. Both Z16 and 7250-14-1 couldn’t restore the fertility of CMS-T, and partly restore the fertility for CMS-S. Genetic analysis showed that the fertility restoration was controlled by a pair of dominant genes for Z16 when crossed with C478 or C Huangzaosi. But for 7250-14-1, the fertility restoration was controlled by a pair of dominant genes for C Huangzaosi, and two pairs of complementary dominant genes for C478. Both of the restorer genes for Z16 and 7250-14-1 were mapped on the short arm of chromosome 8 by molecular markers. For Z16, it was mapped within a physical distance of 494 kb from the marker B-1 to the end of the chromosome, and for 7250-14-1, it was located between B-1 and Chr8-86080, with physical distance of 249 kb. This study not only provides some information for the practical application of Z16 and 7250-14-1, but also lays a foundation for the cloning and functional analysis of restorer genes.

关 键 词：玉米 细胞质雄性不育 恢复基因 分子标记定位 

分 类 号：S513[农业科学—作物学]