背部经脉手法对PCPA失眠大鼠的行为学影响  

The impact of back meridians manipulations on insomnia PCPA-treated rats' behavior

作　　者：吉登军 顾非[1] 李云龙 李亚洲[1] 宁元率 王怡超 曹欣地 柳文韬 JI Dengjun;GU Fei;LI Yunlong;LI Yazhou;NING Yuanshuai;WANG Yichao;CAO Xindi;LIU Wentao(Department of Tuina,Spinal Disease Unit,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院脊柱病单元推拿科,上海200437

出　　处：《中国医药导报》2019年第1期12-15,共4页China Medical Herald

基　　金：上海市科学技术委员会科研计划项目(16401932300);上海市名老中医学术经验研究工作室建设项目(ZYSNXD-CC-MZYO04)

摘　　要：目的观察背部经脉手法对对氯苯丙氨酸(PCPA)失眠大鼠的行为学影响。方法运用SPSS 18.0 for Windows软件生成随机数字表,随机函数为Rvuiform,种子号1234。将30只雄性清洁级SD失眠大鼠按随机数字表分为背部经脉手法组(A组)、空白对照组(B组)、药物治疗组(C组),每组10只。分别于PCPA药物造模前、造模后、治疗7 d后这3个时间点采集大鼠行为学数据,包括旷场实验(水平运动和垂直运动水平的运动总数)、力竭游泳实验、鼠尾悬吊实验数据的变化。结果治疗前三组大鼠力竭时间、旷场运动总数、挣扎次数、绝望时间比较,差异均无统计学意义(P> 0.05)。治疗后B组力竭时间及绝望时间均较治疗前降低(P <0.05);A组和C组治疗前后力竭时间及绝望时间比较,差异无统计学意义(P> 0.05)。与B组比较,A组和C组力竭时间均增加,差异有统计学意义(P <0.05);A组与C组力竭时间比较,差异无统计学意义(P> 0.05)。治疗后三组挣扎次数均较治疗前减少,差异有统计学意义(P <0.05)。结论 PCPA失眠大鼠存在行为学异常,可用于推拿治疗失眠的基础实验研究;行为学上,背部经脉手法和安定均可明显减缓失眠大鼠的运动能力、心肺功能和对新环境的探索能力的下降,是推拿手法改善失眠的客观体现。Objective To observe the impact of back meridians manipulations on insomnia para-chlorophenylalanine(PCPA)-treated rats′behavior.Methods The random number table was generated by software SPSS 18.0 for Windows.The random function was Rvuiform and the seed number was 1234.According to the random number table,30 male insomnia SD rats were divided into back meridians manipulation group(group A),blank control group(group B),drug treatment group(group C),with 10 rats in each group.Before and after the PCPA was successfully injected to the rats,7 days after giving the corresponding interventions to each group,there were three time points at which data were collected from the rats′behavior,including the data changes in the open field test(the total number of horizontal movements and vertical movements),exhaustive swimming test and tail suspension test.Results Before treatment,the differences among the three groups in exhaustive swimming time,total number of movements,struggling frequency and despair time were not statistically significant(P>0.05).After treatment,the exhaustive swimming time and despair time of group B were lower than those before treatment(P<0.05);comparing group A and group C before and after treatment,the differences on the exhaustive swimming time and despair time were all non-statistically significant(P>0.05).Compared with group B,the exhaustion time of group A and group C were increased,the differences were statistically significant(P<0.05);and there was no statistically significant difference in exhaustion time between group A and group C(P>0.05).After treatment,the struggling frequency of three groups were less than those before treatment,the differences were statistically significant(P<0.05).Conclusion There is abnormality in the behavior of insomnia PCPA-treated rats.It can be used in the basic experimental study tuina treatment for insomnia;in behavioral aspects,back meridians manipulations and Diazepam can both significantly alleviate the decline of movement ability,cardiopulmonary functio

关 键 词：背部经脉手法 失眠 对氯苯丙氨酸 动物实验 行为学 

分 类 号：R24[医药卫生—中医临床基础]