三花龙胆化学成分及抗肿瘤活性研究  

Study on chemical constituents and antitumor activity of Gentiana triflora Pall.

作　　者：周艳丽[1] 王大利[2] 张艳[1] 张宁[1] 李硕熙[1] 吴娇 李杨 ZHOU Yanli;WANG Dali;ZHANG Yan;ZHANG Ning;LI Shuoxi;WU Jiao;LI Yang(Jiamusi College of Heilongjiang University of Chinese Medicine,Heilongjiang Province,Jiamusi 154007,China;Experimental Center of Dalian Children′s Hospital,Liaoning Province,Dalian 116012,China;College of Life Science,Dalian Nationalities University,Liaoning Province,Dalian 116600,China)

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [2]大连市儿童医院中心实验室,辽宁大连116012 [3]大连民族大学生命科学学院,辽宁大连116600

出　　处：《中国医药导报》2019年第1期20-23,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81274035)

摘　　要：目的探讨三花龙胆(Gentiana triflora Pall.)的化学成分及其体外抗肿瘤活性。方法采用95%乙醇对三花龙胆根茎进行回流提取,依次用石油醚、乙酸乙酯、正丁醇进行萃取,萃取物用硅胶柱色谱、制备型高效液相色谱进行分离纯化,根据理化性质和波谱数据对化合物进行结构鉴定;采用四氮唑盐还原法(MTT)评价各化合物的抗肿瘤活性。结果从乙酸乙酯和正丁醇萃取部位分离得到5个化合物,分别鉴定为globuloside A(1)、cornusoside A(2)、cornolactone A(3)、6,9-epi-8-O-Acetylshanziside methyl ester(4)、5,9-epi-Penstemoside(5);抗肿瘤活性实验表明,化合物1对HepG2细胞的体外增殖具有一定的抑制作用,IC50值为6.7μmol/L。结论所有化合物均首次从该植物中分离得到;化合物1对HepG2细胞的增殖具有明显的抑制活性。Objective To investigate the chemical constituents and antitumor activities in vitro of Gentiana triflora Pall.Methods The root of Gentiana triflora Pall. was extracted with 95% ethyl alcohol, which is extracted with petroleum ether, ethyl acetate and n-butanol, successively. The extracts were separated and purified by silica gel column chromatography and preparative high performance liquid chromatography. The compounds were structurally identified according to physicochemical properties and spectral data. The antitumor activities of the compounds were evaluated by microculture tetrozolium(MTT) method. Results Five compounds were isolated from the ethyl acetate and n-butanol extraction sites and identified as globuloside A(1), cornusoside A(2), cornolactone A(3), 6, 9-epi-8-O-Acetylshanziside methyl ester( 4), 5,9-epi-Penstemoside(5); anti-tumor activity experiments showed that Compound 1 had a certain inhibitory effect on the proliferation of HepG2 cells in vitro, with an IC50 value of 6.7 μmol/L. Conclusion All the compounds are isolated from Gentiana triflora Pall. firstly. Compound 1 has significant inhibitory activity on the proliferation of HepG2 cells.

关 键 词：三花龙胆 化学成分 结构鉴定 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]