追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制  

Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells

作　　者：刘敏[1] 刘春亮[2] 梁国强[1] 姚霏[1] 张晓迪[1] 陈婷[1] 袁雅琴 吴士良[2] LIU Min;LIU Chun liang;LIANG Guo qiang;YAO Fei;ZHANG Xiao die;CHEN Ting;YUAN Ya qin;WU Shi liang(Affiliated Suzhou Hospital of TCM to Nanjing University of Chinese Medicine,Suzhou,215009,China;School of Biology and Basic Medical Sciences,Soochow University,Suzhou,215123,China)

机构地区：[1]南京中医药大学附属苏州市中医医院,江苏苏州215009 [2]苏州大学生物化学和分子生物基础医学院,江苏苏州215123

出　　处：《南京中医药大学学报》2019年第1期63-67,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省苏州市科技局项目(SYS201572)

摘　　要：目的基于糖基转移酶β3GnT8探讨追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制。方法通过MTT法观察追毒方对MDA-MB-231耐药细胞增殖的抑制作用,通过转染β3GnT8得到高表达β3GnT8的MDA-MB-231/TAX细胞株,采用Western blot观察糖基转移酶β3GnT8和耐药蛋白BCRP、P-gp的表达,通过凝集素流式细胞术观察转染β3GnT8MDA-MB-231/TAX细胞膜表面β3GnT8的荧光强度。结果追毒方有效成分能明显抑制三阴性乳腺癌MDA-MB-231耐药细胞的增殖,作用72h抑制率达到50.16%,能有效下调β3GnT8和耐药蛋白BCRP、P-gp的表达,并且β3GnT8的表达与BCRP、P-gp呈正相关。结论追毒方可通过下调糖基转移酶β3GnT8,从而降低耐药蛋白BCRP、P-gp的表达,逆转三阴性乳腺癌MDA-MB-231细胞耐药性。OBJECTIVE Based on glycosyltransferaseβ3 GnT8,to investigate the mechanism of reverse effect on triple negative breast cancer MDA-MB-231 drug-resistance cells by Zhuidu Fang.METHODS The inhibition of MDA-MB-31 drug-resistance cells proliferation by Zhuidu Fang was detected by MTT assay.By interference and transfection ofβ3 GnT8,high and low expressedβ3 GnT8 MDA-MB-231/TAX cell lines were obtained.Western blot was used to detect the protein expression ofβ3 GnT8,and drug resistance protein BCRP and P-gp.Lectin flow cytometry was used to detectβ3 GnT8 fluorescence intensity onβ3 GnT8 transfected MDA-MB-231/TAX cells.RESULTS Zhuidu Fang effectively inhibited the proliferation of MDA-MB-231/TAX cells,and the inhibitory rate was 50.16%after 72 h.Zhuidu Fang significantly downregulated the protein expression ofβ3 GnT8,BCRP and P-gp.The protein expression ofβ3 GnT8 showed a positive correlation with BCRP and P-gp.CONCLUSION Zhuidu Fang can reverse the drug-resistance of MDA-MB-231 cell lines,which is associated with the downregulation of the expression ofβ3 GnT8,BCRP and P-gp.

关 键 词：追毒方 三阴性乳腺癌MDAMB231耐药细胞 β3GnT8 逆转耐药 

分 类 号：R285.5[医药卫生—中药学]