基于高分辨率质谱的金欣口服液治疗小鼠RSV感染的血清脂质组学研究  

Lipomics Study of Jinxin Oral Liquid for RSV Infection in Mice Based on UHPLC-Q-Exactive Orbitrap/MS

作　　者：纪建建 杨瑞[1,2,3] 谢彤 沈存思[1,2,3] 林丽丽 汪受传[1,2,3] 单进军 JI Jian jian;YANG Rui;XIE Tong;SHEN Cun si;LIN Li li;WANG Shou chuan;SHAN Jin jun(Jiangsu Key Laboratory of Pediatric Respiratory Disease,Institute of Pediatrics,Nanjing University of Chinese Medicine,Nanjing,210023,China;Medical Metabolomics Center,Nanjing University of Chinese Medicine,Nanjing,210023,China;Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing,210023,China)

机构地区：[1]江苏省儿童呼吸疾病(中医药)重点实验室南京中医药大学中医儿科学研究所,江苏南京210023 [2]南京中医药大学医学代谢组学中心,江苏南京210023 [3]南京中医药大学附属医院,江苏南京210029

出　　处：《南京中医药大学学报》2019年第1期78-84,共7页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省自然科学基金(BK20161573;BK20180825);江苏省"333工程"科研项目(BRA2016427);江苏省高校自然科学研究重大项目(16KJA360002)

摘　　要：目的基于UHPLC-Q-Exactive Orbitrap MS高分辨率质谱,观察RSV诱发的肺炎模型小鼠的血清中脂质代谢的变化,分析金欣口服液对RSV肺炎小鼠中脂质代谢紊乱的调节作用,探讨金欣口服液治疗RSV诱发的病毒性肺炎的潜在机制。方法用RSV诱导小鼠病毒性肺炎,灌胃金欣口服液予以治疗,分别采集对照组、模型组、金欣口服液组小鼠的血清样本,提取脂质成分,采用LC-MS分析,采用主成分分析法(PCA)、聚类分析法(CA)等多元统计方法,对小鼠血清脂质代谢轮廓进行分析,同时进行Kruskal–Wallis test以及fold change法进行统计,寻找差异性脂质。结果 RSV感染导致小鼠肺部炎性细胞浸润及不同程度的肺炎,血清中多种脂质代谢物代谢紊乱,具体表现多数PC、PE出现明显上调,金欣口服液均能改善小鼠肺炎症状且显著恢复血清中异常紊乱的脂质。结论 RSV感染可导致小鼠血清中脂质代谢紊乱,而金欣口服液可一定程度恢复RSV模型中代谢紊乱的脂质,提示金欣口服液可通过调节脂质改善RSV诱发的肺炎。OBJECTIVE Alipidomic method was applied to determine the lipidome in serum of mice based on high-resolution mass spectrometry(Q-Exactive Orbitrap).We investigated the changes of lipids in serum of mice with RSV to explore the regulation of Jinxin oral liquid on lipid metabolism caused by RSV.METHODS The serum samples from the blank group,the RSV model group and Jinxin oral liquid group mice were collected,and the lipids of these samples were extracted and further analyzed by high-resolution mass spectrometry.Multivariate statistical methods such as principal component analysis(PCA)and cluster analysis(CA)were used to analyze the lipid profiles,and the Kruskal-Wallis test and fold change were performed to find the differential lipids.RESULTS The lipids of serum between the blank control group and the RSV model group could be well distinguished,RSV infection in mice with metabolic abnormalities of lipids,the specific performance of the PC,PE was significantly upregulated,Jinxin oral liquid have a certain role in these lipids CONCLUSION RSV infection caused disturbances of lipid in vivo,and Jinxin oral liquid has a certain regulatory effect on the lipid metabolism disorder caused by RSV model.

关 键 词：脂质组学 UHPLC-Q-Exactive Orbitrap/MS 呼吸道合胞病毒 金欣口服液 

分 类 号：R284[医药卫生—中药学]