急性心肌梗死患者体内miR-208表达与心肌损伤、血小板活化的相关性分析  

The expression of microRNA-208 and myocardium jury in patients with acute myocardial infarction

作　　者：曹舸[1] 余惠[1] 范景秀[1] 康慧[1] 张尔永[1] CAO Ge;YU Hui;FAN Jingxiu;KANG Hui;ZHANG Eryong(Department of Cardiovascular Surgery, West China Hospital, Sichuan University,Chendu 610041,China)

机构地区：[1]四川大学华西医院心脏大血管外科,成都610041

出　　处：《疑难病杂志》2019年第1期23-26,共4页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的分析急性心肌梗死(AMI)患者血液miR-208表达变化与心肌损伤、血小板活化的相关性。方法选择2014年6月—2017年12月四川大学华西医院心脏大血管外科确诊为AMI的患者64例作为AMI组,另取同期进行健康体检的志愿者52例作为对照组。测定2组对象血浆miR-208a、miR-208b的表达量以及血清心肌损伤标志物、血小板活化指标的含量。结果与对照组比较,AMI组患者血浆miR-208a的表达量以及血清cTnI、CK-MB、MDA、ET-1、P-selectin、PAC-1、TXB2、PAF、Lp-PLA2的含量显著升高(t/P=31. 309/0. 000、50. 682/0. 000、40. 876/0. 000、36.484/0. 000、36. 342/0.000、33. 857/0. 000、22. 940/0. 000、20. 305/0. 000、23. 464/0. 000、11.698/0. 000),而血浆miR-208b的表达量无显著变化(t/P=1. 607/0.111);AMI组患者血浆miR-208a的表达量与cTnI、CK-MB、MDA、ET-1、P-selectin、PAC-1、TXB2、PAF、Lp-PLA2的含量呈正相关(r/P=0. 683/0. 004、0. 626/0. 007、0. 596/0. 016、0. 653/0. 005、0.585/0.018、0. 703/0.002.、0. 495/0.022、0. 531/0.019、0.628/0.009)。结论 AMI患者外周血中miR-208a表达增多,且与心肌损伤、血小板活化程度相关。Objective To analyze the correlation between the expression of microRNA-208 and myocardial injury and platelet activation in patients with acute myocardial infarction (AMI). Methods From June 2014 to December 2017, 64 patients with AMI diagnosed by cardiac and macrovascular surgery in West China Hospital of Sichuan University were selected as the AMI group, and 52 volunteers who underwent health examination during the same period were selected as the control group. The expression levels of plasma microRNA-208a and microRNA-208b and the levels of serum myocardial damage markers and platelet activation markers were measured. Results Compared with the control group, the levels of plasma miR208a expression and serum cTnI, CK-MB, MDA, ET-1, P-selectin, PAC-1, TXB2, PAF, Lp-PLA2 in AMI group significantly increased( t/P =31.309/0.000, t/P =50.682/0.000, t/P =40.876/0.000, t/P =36.484/0.000, t/P =36.342/0.000, t/P =33.857/0.000, t/P =22.940/0.000, t/P =20.305/0.000, t/P =23.464/0.000, t/P =11.698/0.000), there was no significant change in the expression of plasma microRNA-208b ( t/P =1.607/0.111). The expression of plasma microRNA 208a in AMI group was positively correlated with cTnI, CK-MB, MDA, MDA, ET-1, P-selectin, PAC-1, TXB2, PAF, Lp-PLA2 ( r/P =0.683/0.004, r/P =0.626/0.007, r/P =0.596/0.016, r/P =0.653/0.005, r/P =0.585/0.018, r/P =0.703/0.002, r/P =0.495/0.022, r/P =0.531/0.019, r/P =0.628/0.009). Conclusion The expression of microRNA-208a in peripheral blood of patients with AMI increased, which was related to the degree of myocardial injury and platelet activation.

关 键 词：心肌梗死 急性 miR-208 心肌损伤 血小板活化 相关性 

分 类 号：R542.22[医药卫生—心血管疾病]