铜绿假单胞菌重组Bb-OprH疫苗诱导的保护力及其细胞免疫应答  

Protective effect and cellular immune response induced by recombinant Bb-OprH vaccine of Pseudomonas aeruginosa

作　　者：江帆 李文桂[1] JIANG Fan;LI Wengui(Institute of Infectious and Parasitic Diseases,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《分子影像学杂志》2019年第1期63-66,共4页Journal of Molecular Imaging

基　　金：重庆市科委地方病重大专项基金项目(2008AB5055;2008AB5008;2008AB5054)

摘　　要：目的探讨铜绿假单胞菌(Pa)重组两歧双歧杆菌(rBb)-OprH疫苗免疫及Pa PAO1株攻击后,诱导的小鼠保护力、脾细胞增殖、亚群及凋亡变化情况。方法将疫苗口服灌胃免疫Balb/c鼠,在首免后4周用5×10~7 CFU的PAO1株滴鼻攻击。攻击后2周杀鼠取脾,计数小鼠肺细胞负荷,MTT检测小鼠脾细胞增殖,流式细胞术检测小鼠脾细胞亚群及脾细胞凋亡率。结果疫苗免疫及PAO1株攻击后,小鼠肺细菌负荷减少,脾细胞增殖、CD4^+T细胞明显增加,而脾细胞凋亡明显减少。结论铜绿假单胞菌重组Bb-OprH疫苗可诱导铜绿假单胞菌感染小鼠产生保护性细胞免疫应答。Objective To investigate protection and changes of splenocyte proliferation,apoptosis and subsets after immunization with recombinant Bifidobacterium(rBb)-OprH vaccine of Pseudomonas aeruginosa(Pa).Methods Balb/c mice were vaccinated intragastrically with the vaccine and challenged by Pa PAO1 strain intranasally at the 4th week after the first immunization.The mice were sacrificed two weeks after the challenge to isolate their lungs and spleens,and the pulmonary bacterial loads were counted.MTT assay was used to detect splenocyte proliferation,while apoptic rate and subsets of splenocytes were measured by flow cytometry.Results After immunization with the vaccine and challenged by Pa PAO1 strain,the bacterial loads in the lungs decreased,while the splenocyte proliferation and the number of CD4+T cells increased.The apoptic rate decreased.Conclusion The recombinant Bb-OprH vaccine can induce a protective cellular immune response in mice against the Pseudomonas aeruginosa.

关 键 词：铜绿假单胞菌 重组Bb-OprH疫苗 脾细胞增殖 脾细胞亚群 脾细胞凋亡 

分 类 号：R392[医药卫生—免疫学]