miR-378a-5p下调人肝脏星形细胞系LX-2中鞘胺醇激酶1的表达  

miR-378a-5p down-regulates SphK1 expression in LX-2

作　　者：祁长波 常娜 杨琳[1] 李丽英[1] Qi Changbo;Chang Na;Yang Lin;Li Liying(Department of Cell Biology,Municipal Laboratory for Liver Protection and Regulation of Regeneration,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学细胞生物学系‘肝脏保护与再生调节’北京市重点实验室,北京100069

出　　处：《首都医科大学学报》2019年第1期94-100,共7页Journal of Capital Medical University

基　　金：国家自然科学基金(81770603);北京市自然科学基金(7172019)~~

摘　　要：目的预测、筛选并探究是否存在特定的microRNA能够影响人肝脏星形细胞LX-2中鞘胺醇激酶1(sphingosine kinase1,SphK1) mRNA的表达。方法采用生物信息学数据库预测microRNA,采用实时荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,RT-qPCR)检测SphK1 mRNA的表达。结果预测筛选出的miR-378a-5p、miR-92a-2-5p和miR-708-5p中,只有miR-378a-5p能够抑制LX-2中由转化生长因子β1 (transforming growth factor-β1,TGF-β1)介导的SphK1 mRNA表达上调这一过程。而在LX-2中转染miR-378a-5p的抑制剂阻断其作用后,SphK1 mRNA的表达上调。结论 miR-378a-5p参与LX-2中Sph K1 mRNA表达的调控,并且能够抑制由TGF-β1介导的SphK1 mRNA表达上调。Objective To investigate whether microRNA could regulate the expression of sphingosine kinase 1( SphK1) mRNA in LX-2 cells. Methods Prediction of microRNAs was performed on bioinformatics sites. Real-time quantitative polymerase chain reaction( RT-qPCR) was applied to detect the expression of SphK1 mRNA in LX-2 cells. Results Only miR-378 a-5 p,not miR-92 a-2-5 p or miR-708-5 p,could suppress the increase of Sph K1 mRNA expression caused by transforming growth factor-β1( TGF-β1) in LX-2. And the expression of SphK1 mRNA was up-regulated when the inhibitor of miR-378 a-5p was applied in LX-2 cells. Conclusion miR-378 a-5p took part in the regulation of SphK1 mRNA expression in LX-2,and it could down-regulate the increase of SphK1 mRNA expression caused by TGF-β1.

关 键 词：miR-378a-5p 鞘胺醇激酶1 转化生长因子Β1 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]