离子液体[C_8mim]Cl对泥鳅肝脏的氧化损伤效应  

Oxidative damage effect of ionic liquids [C_8mim]Cl on the liver of loach

作　　者：杜启艳[1] 王亚星[1] 南平[1] 常重杰[1] Du Qiyan;Wang Yaxing;Nan Ping;Chang ZhongJie(College of Life Science,Henan Normal University,Xinxiang 453007,China)

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2019年第1期88-92,共5页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(U1804116);河南省重点科技攻关项目(172102210048;172102110098)

摘　　要：以泥鳅为实验材料,研究离子液体氯化1-辛基-3-甲基咪唑([C8mim]Cl)对其肝脏的氧化损伤效应.结果显示:各浓度组中活性氧(ROS)含量在染毒1d、4d和8d后与对照组相比显著升高,1d时含量达到峰值;脂质过氧化物(LPO)含量在染毒1d时其低、中、高浓度组与对照组相比显著升高,随着暴露时间的延长,在染毒4d和8d时各浓度组LPO含量下降并低于对照组水平;谷丙转氨酶(GPT)含量与对照组相比在低、中、高不同浓度组染毒1d、4d和8d后,均显著下降;谷胱甘肽还原酶(GR)活力在染毒1d、4d和8d后各浓度组与对照组相比均呈现出显著下降趋势,在第8d时,达到最低水平.结果表明离子液体[C8mim]Cl对泥鳅肝脏有明显的氧化损伤效应.本研究结果对于人们正确认识"绿色溶剂"离子液体的安全性及其安全使用具有重要参考价值.In this paper,we studied oxidative damage effect of the ionic liquid 1-octyl-3-methyl imidazole chloride([C 8mim]Cl)on the loach livers.Results showed that compared with control group:Reactive oxygen species(ROS)content significantly increased,and the highest level was at 1 d.Compared with control group,content of lipid peroxide(LPO)at 1 d significantly increased.With increasing exposures time,the content of LPO level decreased and was lower than the control group in 4 d or 8 d.Compared with control group,the content of GPT,were significantly decreased at 1 d,4 d and 8 d after exposed to Ionic Liquids.Glutathione reductase(GR)activity showed a significant declining trend,and the lowest level was at 8 d.These results showed that the ionic liquid[C 8mim]Cl had obvious oxidative damage effects on the liver of loach.The results of this paper have important reference for people to proply understand the safety and correct use of the"green solvent"ionic liquid.

关 键 词：离子液体 泥鳅 氧化损伤 肝脏 

分 类 号：R946.88[医药卫生—药剂学]