牛病毒性腹泻病毒检测技术研究进展  

Progress on Detection Methods of Bovine Viral Diarrhea Virus

作　　者：于志超 陈林军 赵治国 崔强 延涵 王海艳 赵林立 李刚 郭文丽 YU Zhi-chao;CHEN Lin-jun;ZHAO Zhi-guo;CUI Qiang;YAN Han;WANG Hai-yan;ZHAO Lin-li;LI Gang;GUO Wen-li(Inner Mongolia Entry-exit Inspection and Quarantine Bureau,Hohhot,Inner Mongolia,010020,China;Erenhot Center for Disease Control and Prevention,Erenhot,Inner Mongolia,012600,China)

机构地区：[1]内蒙古出入境检验检疫局检验检疫技术中心,内蒙古呼和浩特010020 [2]二连浩特市疾病预防控制中心,内蒙古二连浩特012600

出　　处：《动物医学进展》2019年第1期93-97,共5页Progress In Veterinary Medicine

基　　金：国家质检总局科技计划项目(2017IK102)

摘　　要：牛病毒性腹泻(BVD)是由牛病毒性腹泻病毒(BVDV)所致牛的一种接触性传染病,呈世界性流行,该病主要表现为腹泻、急慢性黏膜感染、繁殖障碍等,对畜牧业危害严重,是国际贸易中动物检疫的重要疫病之一。快速准确的检测手段,有利于该传染病的早期发现和及时控制。目前,用于检测牛病毒性腹泻病毒的方法很多,论文对近年来国内外应用的等温核酸扩增技术(IAT)、重组酶聚合酶扩增技术(RPA)、指数富集配体系统进化技术(SELEX)、多重PCR技术、多重连接探针扩增技术(MLPA)、RT-PCR抗原酶联免疫吸附试验(ELISA)和间接免疫荧光试验(IFA)等进行简要介绍,为我国动物检疫工作提供参考。Bovine viral diarrhea is caused by bovine viral diarrhea virus,which manifests many symptoms,such as diarrhea,acute and chronic mucosal infection and reproductive disturbance.It is seriously harms to animal husbandry and also one of the important animal diseases of animal quarantine in international trade.Rapid and accurate detection is conducive to early detection and control of this disease.At present, there are many methods for detecting bovine viral diarrhea virus.In this paper,we introduced the detection technologies including IAT,RPA,SELEX,multiplex PCR,MLPA,RT-PCR ELISA and IFA,in order to provide reference for rapid and highly effective detecting techniques in the work of animal inspection and quarantine.

关 键 词：牛病毒性腹泻病毒 动物检疫 病原学 血清学 

分 类 号：S852.659.6[农业科学—基础兽医学] S854.43[农业科学—兽医学]