某羊场和田羊与策勒黑羊无浆体季节性感染PCR检测  

PCR Detection of Anaplasma Prevalence in Hotan Sheep and Qira Black Sheep in Various Seasons

作　　者：严勇 赵爱云[1] 李姚旭 蒋维东[1] 石长青[1] 张宽宽 关贵全[2] 齐萌[1] YAN Yong;ZHAO Ai-yun;LI Yao-xu;JIANG Wei-dong;SHI Chang-qing;ZHNAG Kuan-kuan;GUAN Gui-quan;QI Meng(College of Animal Science,Tarim University,Alar,Xinjiang,843300,China;State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Lanzhou,Gansu,730046,China)

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔843300 [2]中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室,甘肃兰州730046

出　　处：《动物医学进展》2019年第1期141-144,共4页Progress In Veterinary Medicine

基　　金：塔里木大学大学生创新创业训练计划项目(2017016);塔里木大学校长基金项目(TDZKCX201401);塔里木大学兽医学学科建设专项项目(SYXZX201802)

摘　　要：为了解和田羊和策勒黑羊无浆体季节动态感染情况,分别于2015年冬至、2016年春分、夏至和秋分,在策勒县某牧业合作社采集和田羊和策勒黑羊血液样本共120份,应用PCR对无浆体的MSP4基因和16SrRNA基因进行扩增。结果发现,检测绵羊的无浆体总阳性率为99.2%(119/120),以绵羊无浆体(Anaplasma ovis)和嗜吞噬无浆体(A.phagocytophilum)混合感染为主,占阳性样本比例为61.3%(73/119),A.ovis和A.phagocytophilum占阳性样本比例分别为31.1%(37/119)和7.6%(9/119)。在不同季节,和田羊和策勒黑羊无浆体均普遍感染,差异不显著(P>0.05)。结果显示,新疆策勒县某羊场绵羊的无浆体病全年均可流行,应引起人们重视。To understand the prevalence of Anaplasma in Hotan sheep and Qika black sheep in various seasons,120 blood samples of Hotan sheep and Qika black sheep were collected from a sheep farm in Qika county in the Winter Solstice in 2015,the Spring Equinox,the Summer Solstice and the Autumn Equinox in 2016,respectively.All samples were examined by PCR based on the MSP4 and 16S rRNA genes of Anaplasma .The overall positive rate of Anaplama in sheep was 99.2%(119/120).Co-infections with Anaplasma ovis and A.phagocytophilum were predominant and the proportion of them in positive samples was 61.3%(73/119).The proportion of A.ovis and A.phagocytophilum in positive samples was 31.1%(37/119) and 7.6%(9/119),respectively.The Anaplasma infections in Hotan sheep and Qika black sheep were common in various seasons,and the difference was not significant ( P >0.05).The result showed that Anaplasma infections in sheep can occur throughout the year in Qika county Xinjiang,which should be noticed.

关 键 词：无浆体 绵羊 感染 聚合酶链反应 

分 类 号：S858.74[农业科学—临床兽医学] S852.33[农业科学—兽医学]